, the French National Research Agency (ANR) as part of the second Investissements d'Avenir " programme (light4deaf, ANR-15-RHUS-0001), ANR- HearInNoise-(ANR-17-CE16-0017, LHW-Stiftung, FAUN Stiftung (Suchert Foundation), LABEX Life-senses, 2010.
, LABX-65), and a grant from the Fondation Pour l'Audition to DD (2015-APA Research Grant)
, Address correspondence to: Didier Dulon, p.75015
, Unité de Génétique et Physiologie de l'Audition, Institut Pasteur, 25 rue Dr Roux
, Philadelphia Pennsylvania, USA) Microinjections were performed on the left ear as previously described (46) For quantification of in vivo injections of
, Then, in each case, we manually counted all visible inner and outer hair cells and determined the percentage of GFP-positive cells. Statistics. For in vivo recordings (data expressed as mean ± SD or SEM as indicated), statistical significance of the differences observed was tested with Student's t test, 2-way ANOVA coupled to the Bonferroni post hoc test (2-way ANOVA), or 2-tailed unpaired t test with Welch's correction using Prism software (GraphPad) For ex vivo recordings, the data (expressed as mean ± SEM) were analyzed with Origin (Microcal) and Igor software (WaveMetrics) Statistical significance is indicated in the figures, GFP
, 05, **P < 0.01, ***P < 0.001. Study approval. All the experiments on mice were carried out according to protocols approved by the Animal Use Committees of INSERM, CEEA50), and Institut Pasteur
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