Conformational switch of harmonin, a submembrane scaffold protein of the hair cell mechanoelectrical transduction machinery - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue FEBS Letters Année : 2017

Conformational switch of harmonin, a submembrane scaffold protein of the hair cell mechanoelectrical transduction machinery

Bruno Baron
  • Fonction : Auteur
  • PersonId : 757146
  • IdRef : 163546797

Résumé

Mutations in the gene encoding harmonin, a multi-PDZ domain-containing submembrane protein, cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss). The structure of the protein and biological activities of its three different classes of splice isoforms (a, b, and c) remain poorly understood. Combining biochemical and biophysical analyses , we show that harmonin-a1 can switch between open and closed conforma-tions through intramolecular binding of its C-terminal PDZ-binding motif to its N-terminal supramodule NTD-PDZ1 and through a flexible PDZ2-PDZ3 linker. This conformational switch presumably extends to most harmonin iso-forms, and it is expected to have an impact on the interaction with some binding partners, as shown here for cadherin-related 23, another component of the hair cell mechanoelectrical transduction machinery.
Fichier principal
Vignette du fichier
FEB2-591-2299.pdf (1.11 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

pasteur-01858477 , version 1 (20-08-2018)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Amel Bahloul, Elise Pepermans, Bertrand Raynal, Nicolas Wolff, Florence Cordier, et al.. Conformational switch of harmonin, a submembrane scaffold protein of the hair cell mechanoelectrical transduction machinery. FEBS Letters, 2017, 591 (15), pp.2299 - 2310. ⟨10.1002/1873-3468.12729⟩. ⟨pasteur-01858477⟩
908 Consultations
246 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More