Skip to Main content Skip to Navigation
Journal articles

Circulating Aspergillus fumigatus DNA Is Quantitatively Correlated to Galactomannan in Serum

Abstract : The performance of antigen galactomannan (GM) for diagnosing invasive aspergillosis (IA) is hampered by the occurrence of false-positive results. Quantitative PCR has been proposed to improve the diagnosis of IA. Therefore, we analyzed the value of performing a PCR test to the GM-positive serum sample. Using a quantitative PCR assay specific for Aspergillus fumigatus 28S ribosomal DNA, we retrospectively tested 422 GM-positive (Platelia Bio-Rad kit) serum samples collected over 1 year from 147 patients. The cases were classified based on EORTC criteria as "proven," "probable," and "no-IA" before availability of the PCR results. After exclusion of 65 samples for non-reproducibility of GM positivity (n = 62) or PCR inhibition (n = 3), 75 (21.0%) of the remaining 357 samples were PCR-positive. GM and fungal DNA showed a significantly positive correlation (p < 0.0001, R2 = 0.27, slope = 0.98 ± 0.19). At least one PCR-positive result was observed in 63.3% (31/49) of IA patients and in 13.2% (13/98) of non-IA patients (p < 0.0001). The PCR positivity was also associated with the presence of other microbiological criteria among the 44 patients with IA and complete mycological workup (p = 0.014), as well as a higher mortality rate at six months among the 135 patients with hematological conditions (p = 0.0198). Overall, we found a quantitative correlation between serum GM and circulating DNA with an increased likelihood of IA when both were positive. A PCR-positive result also supported a higher fungal load when GM was already positive. We advocate adding a PCR test for every confirmed GM-positive serum sample.
Document type :
Journal articles
Complete list of metadatas

Cited literature [43 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01851829
Contributor : Reine Bouyssie <>
Submitted on : Tuesday, July 31, 2018 - 9:46:45 AM
Last modification on : Wednesday, August 19, 2020 - 11:18:29 AM
Long-term archiving on: : Thursday, November 1, 2018 - 12:36:47 PM

File

aspergillus.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Alexandre Alanio, Jean Menotti, Maud Gits-Muselli, Samia Hamane, Blandine Denis, et al.. Circulating Aspergillus fumigatus DNA Is Quantitatively Correlated to Galactomannan in Serum. Frontiers in Microbiology, Frontiers Media, 2017, 8, pp.2040. ⟨10.3389/fmicb.2017.02040⟩. ⟨pasteur-01851829⟩

Share

Metrics

Record views

454

Files downloads

896