Skip to Main content Skip to Navigation
Journal articles

Neuralized regulates Crumbs endocytosis and epithelium morphogenesis via specific Stardust isoforms

Abstract : Crumbs (Crb) is a conserved determinant of apical membrane identity that regulates epithelial morphogenesis in many developmental contexts. In this study, we identify the Crb complex protein Stardust (Sdt) as a target of the E3 ubiquitin ligase Neuralized (Neur) in Drosophila melanogaster. Neur interacts with and down-regulates specific Sdt isoforms containing a Neur binding motif (NBM). Using a CRISPR (clustered regularly interspaced short palindromic repeats)-induced deletion of the NBM-encoding exon, we found that Sdt is a key Neur target and that Neur acts via Sdt to down-regulate Crb. We further show that Neur promotes the endocytosis of Crb via the NBM-containing isoforms of Sdt. Although the regulation of Crb by Neur is not strictly essential, it contributes to epithelium remodeling in the posterior midgut and thereby facilitates the trans-epithelial migration of the primordial germ cells in early embryos. Thus, our study uncovers a novel regulatory mechanism for the developmental control of Crb-mediated morphogenesis.
Document type :
Journal articles
Complete list of metadatas

Cited literature [67 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01799354
Contributor : Doriane Thouvenot <>
Submitted on : Thursday, May 24, 2018 - 4:02:06 PM
Last modification on : Monday, January 13, 2020 - 5:08:18 PM
Long-term archiving on: : Saturday, August 25, 2018 - 2:10:41 PM

File

1405.full.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution - NonCommercial - ShareAlike 4.0 International License

Identifiers

Citation

Gantas Perez-Mockus, Vanessa Roca, Khalil Mazouni, François Schweisguth. Neuralized regulates Crumbs endocytosis and epithelium morphogenesis via specific Stardust isoforms. Journal of Cell Biology, Rockefeller University Press, 2017, 216 (5), pp.1405 - 1420. ⟨10.1083/jcb.201611196⟩. ⟨pasteur-01799354⟩

Share

Metrics

Record views

467

Files downloads

425