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Journal articles
Neuralized regulates Crumbs endocytosis and epithelium morphogenesis via specific Stardust isoforms
Abstract : Crumbs (Crb) is a conserved determinant of apical membrane identity that regulates epithelial morphogenesis in many developmental contexts. In this study, we identify the Crb complex protein Stardust (Sdt) as a target of the E3 ubiquitin ligase Neuralized (Neur) in Drosophila melanogaster. Neur interacts with and down-regulates specific Sdt isoforms containing a Neur binding motif (NBM). Using a CRISPR (clustered regularly interspaced short palindromic repeats)-induced deletion of the NBM-encoding exon, we found that Sdt is a key Neur target and that Neur acts via Sdt to down-regulate Crb. We further show that Neur promotes the endocytosis of Crb via the NBM-containing isoforms of Sdt. Although the regulation of Crb by Neur is not strictly essential, it contributes to epithelium remodeling in the posterior midgut and thereby facilitates the trans-epithelial migration of the primordial germ cells in early embryos. Thus, our study uncovers a novel regulatory mechanism for the developmental control of Crb-mediated morphogenesis.
Cited literature [67 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01799354
Contributor : Doriane Thouvenot <>
Submitted on : Thursday, May 24, 2018 - 4:02:06 PM
Last modification on : Tuesday, December 8, 2020 - 10:57:40 AM
Long-term archiving on: : Saturday, August 25, 2018 - 2:10:41 PM
Contributor : Doriane Thouvenot <>
Submitted on : Thursday, May 24, 2018 - 4:02:06 PM
Last modification on : Tuesday, December 8, 2020 - 10:57:40 AM
Long-term archiving on: : Saturday, August 25, 2018 - 2:10:41 PM
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