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Journal articles
Characterization of botulinum neurotoxin type A subtypes by immunocapture enrichment and liquid chromatography–tandem mass spectrometry
Abstract : Botulinum neurotoxins (BoNT) are divided into seven toxinotypes based on their immunological properties and each toxinotype contains several subtypes according to their amino acid sequences. Here, we designed a mass spectrometry method able to identify BoNT/A subtypes in complex matrices including crude culture supernatants, food, and environmental samples. Peptides from BoNT light chain (L) specific to the subtypes BoNT/A1 to A3 and BoNT/A5 to A8 were identified. The method consists of an immunocapture step with antibodies specific to BoNT/A L chains followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a triple quadrupole mass spectrometer (QqQ) in multiple reaction monitoring (MRM) mode. BoNT/A subtypes were correctly identified in culture supernatants and in tap water or orange juice samples with a limit of detection of 20 to 150 mouse lethal doses (MLD) and with a lower sensitivity in serum samples.
Cited literature [48 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01783532
Contributor : Floran Bidault <>
Submitted on : Tuesday, July 31, 2018 - 4:26:58 PM
Last modification on : Monday, June 1, 2020 - 2:34:08 PM
Long-term archiving on: : Thursday, November 1, 2018 - 1:27:17 PM
Contributor : Floran Bidault <>
Submitted on : Tuesday, July 31, 2018 - 4:26:58 PM
Last modification on : Monday, June 1, 2020 - 2:34:08 PM
Long-term archiving on: : Thursday, November 1, 2018 - 1:27:17 PM
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