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DOI : 10.1371/journal.pone.0069042

, Supplementary Figure 4. Cells of the intestinal epithelium which accumulated HCcB, were not immunoreactive with antibodies ITF and cytokeratin18 specific of goblet cells

, Cy3 (100 µg) was injected into mouse ligated jejuno-ileal loop After 10 min incubation the intestinal samples were prepared as described in Fig. 1. The preparations were co-stained with antibodies anti-ITF (green) or anti-cytokeratin18 (green), as well as anti E cadherin (ECCD2) (white) and Hoechst (blue)

, Supplementary Figure 5 The ganglioside GM1 does not impair HCcB entrys into the intestinal mucosa HCcB-Cy3 (70 µg) was injected into ligated jejuno-ileal loop (A) or incubated with a 10-fold excess of GM1 for 20 min at room temperature prior to injection into intestinal loop (B) After 15 min incubation the intestinal samples were prepared as described in Fig

, Supplementary Figure 6 Visualization of fluorescent HCcB (10 µg) in the mouse intestine following its injection into the intestinal lumen. HCcB-Cy3 (10 µg) in Dulbecco's modified Eagle's medium was injected into ligated jejuno-ileal loop of anesthetized mice

, After 20 min incubation the intestinal samples were prepared as described in Fig

, Preparations were co-stained with antibodies anti E-cadherin (ECCD2) and Hoechst (blue)

, Scale bars = 10 µm