Skip to Main content Skip to Navigation
Journal articles

DNA electroporation in rabbits as a method for generation of high-titer neutralizing antisera

Abstract : Raising high titer antibodies in animals is usually performed by protein immunization, which requires the long and sometimes difficult step of production of the recombinant protein. DNA immunization is an alternative to recombinant proteins, only requiring the building of an eukaryotic expression plasmid. Thanks to efficient DNA delivery techniques such as in vivo electroporation, DNA vaccination has proven useful the last few years. In this work, we have shown that it is possible to raise very high antibody titers in rabbit by DNA electroporation of an antigen encoding plasmid in the skeletal muscle with the right set of electrodes and rabbit strain. In a model of botulinum toxins types A and E, the neutralizing titers obtained after three treatments were high enough to fit the European Pharmacopeia, while it did not for type B toxin. Furthermore, the raised antibodies have high avidity and are suitable for in vitro and in vivo immunodetection of proteins.
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01766305
Contributor : Floran Bidault <>
Submitted on : Friday, April 13, 2018 - 2:40:48 PM
Last modification on : Tuesday, September 22, 2020 - 3:58:42 AM

Links full text

Identifiers

Citation

Aurore Burgain, Alice Rochard, Capucine Trollet, Christelle Mazuet, Michel Popoff, et al.. DNA electroporation in rabbits as a method for generation of high-titer neutralizing antisera. Human Vaccines & Immunotherapeutics, Taylor & Francis, 2013, 9 (10), pp.2147 - 2156. ⟨10.4161/hv.25192⟩. ⟨pasteur-01766305⟩

Share

Metrics

Record views

439