Skip to Main content Skip to Navigation
New interface
Journal articles

DNA electroporation in rabbits as a method for generation of high-titer neutralizing antisera

Abstract : Raising high titer antibodies in animals is usually performed by protein immunization, which requires the long and sometimes difficult step of production of the recombinant protein. DNA immunization is an alternative to recombinant proteins, only requiring the building of an eukaryotic expression plasmid. Thanks to efficient DNA delivery techniques such as in vivo electroporation, DNA vaccination has proven useful the last few years. In this work, we have shown that it is possible to raise very high antibody titers in rabbit by DNA electroporation of an antigen encoding plasmid in the skeletal muscle with the right set of electrodes and rabbit strain. In a model of botulinum toxins types A and E, the neutralizing titers obtained after three treatments were high enough to fit the European Pharmacopeia, while it did not for type B toxin. Furthermore, the raised antibodies have high avidity and are suitable for in vitro and in vivo immunodetection of proteins.
Complete list of metadata
Contributor : Floran Bidault Connect in order to contact the contributor
Submitted on : Friday, April 13, 2018 - 2:40:48 PM
Last modification on : Tuesday, November 15, 2022 - 3:59:38 AM

Links full text



Aurore Burgain, Alice Rochard, Capucine Trollet, Christelle Mazuet, Michel Popoff, et al.. DNA electroporation in rabbits as a method for generation of high-titer neutralizing antisera. Human Vaccines & Immunotherapeutics, 2013, 9 (10), pp.2147 - 2156. ⟨10.4161/hv.25192⟩. ⟨pasteur-01766305⟩



Record views