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Characterization of the enzymatic activity of Clostridium perfringens TpeL

Abstract : TpeL is a toxin produced by Clostridium perfringens which belongs to the large clostridial glucosylating toxin family. It was shown that TpeL modifies Ras using UDP-glucose or UDP-N-acetylglucosamine as cosubstrates (Guttenberg et al., 2012; Nagahama et al., 2011). We confirmed that TpeL preferentially glucosaminates the three isoforms of Ras (cH-Ras, N-Ras, and K-Ras) from UDP-N-acetylglucosamine and to a lower extent Rap1a and R-Ras3, and very weakly Rac1. In contrast to previous report, we observed that Ral was not a substrate of TpeL. In addition, we confirmed by in vitro glucosylation and mass spectrometry that TpeL modifies cH-Ras at Thr35.
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Serge Pauillac, Jacques d'Allayer, Pascal Lenormand, Jean Claude Rousselle, Philippe Bouvet, et al.. Characterization of the enzymatic activity of Clostridium perfringens TpeL. Toxicon, 2013, 75, pp.136 - 143. ⟨10.1016/j.toxicon.2013.07.003⟩. ⟨pasteur-01766020⟩



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