Skip to Main content Skip to Navigation
Journal articles

Differential entry of Botulinum Neurotoxin A into neuronal and intestinal cells: an ultrastructural approach

Abstract : Recombinant C-terminus (Hc) of botulinum neurotoxin A (BoNT/A) was used to monitor toxin entry into NG108-15 neuronal cells and m-ICcl2 intestinal cells by both fluorescence and electronic microscopy. BoNT/A Hc entered neuronal cells via a clathrin pathway and intestinal cells mainly via non-clathrin vesicles. Upon incubation at 4°C, BoNT/A Hc gold particles were mainly distributed to neuronal cell periphery and to a lower extent to m-ICcl2 cells. After incubation at 37°C, BoNT/A Hc mostly transited in coated vesicles in NG108-15 cells and in uncoated vesicles of m-ICcl2 cells, which support the transcytotic passage of the toxin.
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01758348
Contributor : Floran Bidault <>
Submitted on : Wednesday, April 4, 2018 - 2:10:16 PM
Last modification on : Tuesday, July 21, 2020 - 11:20:03 PM

Identifiers

Collections

Citation

Aurélie Couesnon, Cesare Colasante, Jordi Molgó, Michel Popoff. Differential entry of Botulinum Neurotoxin A into neuronal and intestinal cells: an ultrastructural approach. The Botulinum Journal, 2010, 1 (4), ⟨10.1504/TBJ.2010.039113⟩. ⟨pasteur-01758348⟩

Share

Metrics

Record views

95