Proteomic analysis of plasma extracellular vesicles reveals mitochondrial stress upon HTLV-1 infection

Patricia Jeannin (1) , Thibault Chaze (2) , Quentin Giai Gianetto (3, 2) , Mariette I Matondo (2) , Olivier Gout (4) , Antoine S Gessain (1) , Philippe Afonso (1)

1 EPVO (UMR_3569 / U-Pasteur_3) - Epidémiologie et Physiopathologie des Virus Oncogènes
2 UTechS MSBio - Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology
3 C3BI - Centre de Bioinformatique, Biostatistique et Biologie Intégrative
4 Fondation Ophtalmologique Adolphe de Rothschild [Paris]

Extracellular vesicles (EVs) can participate in intercellular communication and pathogenesis. EVs contain many cargos, including proteins, and the composition of EVs differs between cell-types and activation levels. Thus, plasma EVs can be used as a biomarker of systemic response to infection and/ or disease progression. In this study, we aimed at describing alterations in the protein content of plasma EVs upon infection with the human T-lymphotropic retrovirus type 1 (HTLV-1). HTLV-1 is the etiological agent of a lymphoproliferative disease (ATL) and a series of inflammatory diseases, including a neurodegenerative inflammatory disease (HAM/TSP). We found that plasma EVs are more abundant and smaller in HTLV-1 asymptomatic carriers or HAM/TSP patients when compared to uninfected healthy donors. Moreover, EVs from HTLV-1 infected donors contain markers of metabolic and mitochondrial stress.

Keywords: Mitochondria; Viral infection

Domains: Life Sciences [q-bio]; Microbiology and Parasitology; Virology

Patricia Jeannin, Thibault Chaze, Quentin Giai Gianetto, Mariette I Matondo, Olivier Gout, et al.. Proteomic analysis of plasma extracellular vesicles reveals mitochondrial stress upon HTLV-1 infection. Scientific Reports, 2018, 8, pp.5170 - 5170. ⟨10.1038/s41598-018-23505-0⟩. ⟨pasteur-01744767⟩