The Composition of the Culture Medium Influences the β-1,3-Glucan Metabolism of Aspergillus fumigatus and the Antifungal Activity of Inhibitors of β-1,3-Glucan Synthesis

Cécile Clavaud (1) , Anne Beauvais (1) , Lise Barbin (2) , Hélène Munier-Lehmann (3) , Jean-Paul Latgé (1)

1 Aspergillus
2 CHU Nantes - Centre Hospitalier Universitaire de Nantes
3 Chimie et Biocatalyse - Chemistry and Biocatalysis (group)

In vitro testing of Aspergillus fumigatus susceptibility to echinocandins has always been a challenge. Using a simple and quick colorimetric method to analyze the activity of inhibitors of β-1,3-glucan synthesis, we found that the composition of the culture medium significantly influences glucan synthesis and consequently the antifungal properties of inhibitors of β-1,3-glucan synthesis when they are tested alone or in combination with chitin synthase inhibitors.

Domaines: Sciences du Vivant [q-bio]; Mycologie

Cécile Clavaud, Anne Beauvais, Lise Barbin, Hélène Munier-Lehmann, Jean-Paul Latgé. The Composition of the Culture Medium Influences the β-1,3-Glucan Metabolism of Aspergillus fumigatus and the Antifungal Activity of Inhibitors of β-1,3-Glucan Synthesis. Antimicrobial Agents and Chemotherapy, 2012, 56 (6), pp.3428 - 3431. ⟨10.1128/AAC.05661-11⟩. ⟨pasteur-01725799⟩