Skip to Main content Skip to Navigation
Journal articles

Mutational analysis of the charge selectivity filter of the alpha7 nicotinic acetylcholine receptor.

Abstract : In the alpha7 nicotinic acetylcholine receptors, we analyze the contribution of mutations E237A and V251T, together with the proline insertion P236', in the conversion of the charge selectivity from cationic to anionic. We show that the triple mutant exhibits spontaneous openings displaying anionic selectivity. Furthermore, at position 251, hydrophilic or even negatively charged residues are compatible with an anionic channel. In contrast, the additional proline yields an anionic channel only when inserted between positions 234 and 237; insertion before 234 yields a cationic channel and after 238 alters the receptor surface expression. The coiled 234-238 loop thus directly contributes to the charge selectivity filter of the alpha7 channel.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01718442
Contributor : Ana Cova Rodrigues <>
Submitted on : Tuesday, February 27, 2018 - 2:04:02 PM
Last modification on : Wednesday, October 14, 2020 - 3:59:25 AM

Links full text

Identifiers

Collections

Citation

Pierre-Jean Corringer, Sonia Bertrand, Jean-Luc Galzi, Anne Devillers-Thiéry, Jean-Pierre Changeux, et al.. Mutational analysis of the charge selectivity filter of the alpha7 nicotinic acetylcholine receptor.. Neuron, Elsevier, 1999, 22 (4), pp.831-43. ⟨10.1016/S0896-6273(00)80741-2⟩. ⟨pasteur-01718442⟩

Share

Metrics

Record views

91