Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi - Archive ouverte HAL Access content directly
Journal Articles Biochemical Journal Year : 2000

Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi

Abstract

A new family of glycosylphosphatidylinositol-anchored beta(1-3)glucanosyltransferases (Gelp), recently identified and characterized in the filamentous fungus Aspergillus fumigatus, showed functional similarity to the Gas/Phr/Epd protein families, which are involved in yeast morphogenesis. Sequence comparisons and hydrophobic cluster analysis (HCA) showed that all the Gas/Phr/Epd/Gel proteins belong to a new family of glycosylhydrolases, family 72. We confirmed by site-directed mutagenesis and biochemical analysis that the two conserved glutamate residues (the putative catalytic residues of this family, as determined by HCA) are involved in the active site of this family of glycosylhydrolases.

Dates and versions

pasteur-01711043 , version 1 (16-02-2018)

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Isabelle Mouyna, Michel Monod, Thierry Fontaine, Bernard Henrissat, Barbara Léchenne, et al.. Identification of the catalytic residues of the first family of beta(1-3)glucanosyltransferases identified in fungi. Biochemical Journal, 2000, 347 (3), pp.741-747. ⟨10.1042/bj3470741⟩. ⟨pasteur-01711043⟩
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