Skip to Main content Skip to Navigation
Journal articles

Chitin Synthases with a Myosin Motor-Like Domain Control the Resistance of Aspergillus fumigatus to Echinocandins

Abstract : Aspergillus fumigatus has two chitin synthases (CSMA and CSMB) with a myosin motor-like domain (MMD) arranged in a head-to-head configuration. To understand the function of these chitin synthases, single and double csm mutant strains were constructed and analyzed. Although there was a slight reduction in mycelial growth of the mutants, the total chitin synthase activity and the cell wall chitin content were similar in the mycelium of all of the mutants and the parental strain. In the conidia, chitin content in the ΔcsmA strain cell wall was less than half the amount found in the parental strain. In contrast, the ΔcsmB mutant strain and, unexpectedly, the ΔcsmA/ΔcsmB mutant strain did not show any modification of chitin content in their conidial cell walls. In contrast to the hydrophobic conidia of the parental strain, conidia of all of the csm mutants were hydrophilic due to the presence of an amorphous material covering the hydrophobic surface-rodlet layer. The deletion of CSM genes also resulted in an increased susceptibility of resting and germinating conidia to echinocandins. These results show that the deletion of the CSMA and CSMB genes induced a significant disorganization of the cell wall structure, even though they contribute only weakly to the overall cell wall chitin synthesis.
Document type :
Journal articles
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01709201
Contributor : Nirina Andriakoto <>
Submitted on : Wednesday, February 14, 2018 - 4:36:08 PM
Last modification on : Thursday, May 28, 2020 - 12:58:02 PM

Links full text

Identifiers

Collections

Citation

Cristina Jiménez-Ortigosa, Vishukumar Aimanianda, Laetitia Muszkieta, Isabelle Mouyna, David Alsteens, et al.. Chitin Synthases with a Myosin Motor-Like Domain Control the Resistance of Aspergillus fumigatus to Echinocandins . Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2012, 56 (12), pp.6121 - 6131. ⟨10.1128/AAC.00752-12⟩. ⟨pasteur-01709201⟩

Share

Metrics

Record views

130