Mosquito Rasputin interacts with chikungunya virus nsP3 and determines the infection rate in Aedes albopictus

Jelke J Fros (1) , Corinne Geertsema (1) , Karima Zouache (2) , Jim Baggen (1) , Natalia Domeradzka (1) , Daniël M van Leeuwen (1) , Jacky Flipse (1) , Just P Vlak (1) , Anna-Bella Failloux (2) , Gorben P Pijlman (1)

1 Laboratory of Virology
2 Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors

Background: Chikungunya virus (CHIKV) is an arthritogenic alphavirus (family Togaviridae), transmitted by Aedes species mosquitoes. CHIKV re-emerged in 2004 with multiple outbreaks worldwide and recently reached the Americas where it has infected over a million individuals in a rapidly expanding epidemic. While alphavirus replication is well understood in general, the specific function (s) of non-structural protein nsP3 remain elusive. CHIKV nsP3 modulates the mammalian stress response by preventing stress granule formation through sequestration of G3BP. In mosquitoes, nsP3 is a determinant of vector specificity, but its functional interaction with mosquito proteins is unclear. Methods: In this research we studied the domains required for localization of CHIKV nsP3 in insect cells and demonstrated its molecular interaction with Rasputin (Rin), the mosquito homologue of G3BP. The biological involvement of Rin in CHIKV infection was investigated in live Ae. albopictus mosquitoes.

Keywords: Chikungunya virus; nsP3; Rasputin; Vector competence; Aedes albopictus; G3BP

Domains: Virology

Jelke J Fros, Corinne Geertsema, Karima Zouache, Jim Baggen, Natalia Domeradzka, et al.. Mosquito Rasputin interacts with chikungunya virus nsP3 and determines the infection rate in Aedes albopictus. Parasites & Vectors, 2015, 8 (1), pp.464. ⟨10.1186/s13071-015-1070-4⟩. ⟨pasteur-01678901⟩