Skip to Main content Skip to Navigation
Journal articles

Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice

Abstract : Urinary tract infections (UTI) are extremely common worldwide, incurring significant morbidity and healthcare-associated expenses. Small animal models, which accurately reflect disease establishment and progression, permit dissection of host-pathogen interactions and generation of immunity to infection. In mice, intravesical instillation of uropathogenic E. coli, the causative agent in more than 85% of community acquired UTI, recapitulates many of the stages of infection observed in humans. Until recently, however, UTI could only be modeled in female animals. This limitation has hindered the study of sex-related differences in UTI, as well as other bladder pathologies, such as cancer. Here, we describe a method to instill male mice that allows direct comparison between female and male animals and provide a detailed protocol to assess bladder tissue by flow cytometry as a means to better understand host responses to infection. Together, these approaches will aid in the identification of host factors that contribute to sex biases observed in UTI and other bladder-associated diseases.
Document type :
Journal articles
Complete list of metadatas

Cited literature [23 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01677512
Contributor : Marie-Christine Vougny <>
Submitted on : Thursday, March 1, 2018 - 2:45:21 PM
Last modification on : Thursday, April 2, 2020 - 3:20:09 PM

File

document.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Anna Zychlinsky Scharff, Matthew L. Albert, Molly A Ingersoll. Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice. Journal of visualized experiments : JoVE, JoVE, 2017, pp.54432 - 54432. ⟨10.3791/54432⟩. ⟨pasteur-01677512⟩

Share

Metrics

Record views

233

Files downloads

205