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Journal articles
Dirhamnolipids secreted from Pseudomonas aeruginosa modify anjpegungal susceptibility of Aspergillus fumigatus by inhibiting β1,3 glucan synthase activity.
Abstract : Pseudomonas aeruginosa and Aspergillus fumigatus are the two microorganisms responsible for most of the chronic infections in cystic fibrosis patients. P. aeruginosa is known to produce quorum-sensing controlled rhamnolipids during chronic infections. Here we show that the dirhamnolipids secreted from P. aeruginosa (i) induce A. fumigatus to produce an extracellular matrix, rich in galactosaminogalactan, 1,8-dihydroxynaphthalene (DHN)-and pyo-melanin, surrounding their hyphae, which facilitates P. aeruginosa binding and (ii) inhibit A. fumigatus growth by blocking β1,3 glucan synthase (GS) activity, thus altering the cell wall architecture. A. fumigatus in the presence of diRhls resulted in a growth phenotype similar to that upon its treatment with antifungal echinocandins, showing multibranched hyphae and thicker cell wall rich in chitin. The diRhl structure containing two rhamnose moieties attached to fatty acyl chain is essential for the interaction with β1,3 GS; however, the site of action of diRhls on GS is different from that of echinocandins, and showed synergistic antifungal effect with azoles.
Cited literature [51 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01622299
Contributor : Marie Martin Connect in order to contact the contributor
Submitted on : Tuesday, October 24, 2017 - 11:56:22 AM
Last modification on : Monday, November 15, 2021 - 11:00:05 AM
Contributor : Marie Martin Connect in order to contact the contributor
Submitted on : Tuesday, October 24, 2017 - 11:56:22 AM
Last modification on : Monday, November 15, 2021 - 11:00:05 AM
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