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Enterococcus faecalis promotes innate immune suppression and polymicrobial catheter-associated urinary tract infection

Abstract : Enterococcus faecalis, a member of the human gastrointestinal microbiota, is an opportunistic pathogen associated with hospital-acquired wound, bloodstream, and urinary tract infections. E. faecalis can subvert or evade immune-mediated clearance, although the mechanisms are poorly understood. In this study, we examined E. faecalis-mediated subversion of macrophage activation. We observed that E. faecalis actively prevents NF-κB signaling in mouse RAW264.7 macrophages in the presence of Toll-like receptor agonists and during polymicrobial infection with Escherichia coliE. faecalis and E. coli co-infection in a mouse model of catheter-associated urinary tract infection (CAUTI) resulted in a suppressed macrophage transcriptional response in the bladder compared to E. coli infection alone. Finally, we demonstrated that co-inoculation of E. faecalis with a commensal strain of E. coli into catheterized bladders significantly augmented E. coli CAUTI. Taken together, these results support that E. faecalis suppression of NF-κB-driven responses in macrophages promotes polymicrobial CAUTI pathogenesis especially when co-infected with less virulent or commensal E. coli strains.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01620314
Contributor : Marie-Christine Vougny <>
Submitted on : Friday, October 20, 2017 - 1:32:35 PM
Last modification on : Thursday, April 2, 2020 - 3:26:11 PM

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Brenda Yin Qi Tien, Hwee Mian Sharon Goh, Kelvin Kian Long Chong, Soumili Bhaduri-Tagore, Sarah Holec, et al.. Enterococcus faecalis promotes innate immune suppression and polymicrobial catheter-associated urinary tract infection. Infection and Immunity, American Society for Microbiology, In press, ⟨10.1128/IAI.00378-17⟩. ⟨pasteur-01620314⟩

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