Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

Darragh Duffy; Vincent Rouilly; Cécile Braudeau; Véronique Corbière; Raouf Djebali; Marie-Noelle Ungeheuer; Régis Josien; Samuel T Labrie; Olivier Lantz; Delphine Louis; Eva Martinez-Caceres; Francoise Mascart; Jose G Ruiz de Morales; Catherine Ottone; Lydia Redjah; Nina Salabert-Le Guen; Alain Savenay; Manfred Schmolz; Antoine Toubert; Matthew L Albert

Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

Darragh Duffy ^{1, 2} Vincent Rouilly ² Cécile Braudeau ^{3, 4} Véronique Corbière ⁵ Raouf Djebali ² Marie-Noelle Ungeheuer ^{6, 2} Régis Josien ^{3, 4} Samuel T Labrie ⁷ Olivier Lantz ⁸ Delphine Louis ⁸ Eva Martinez-Caceres ⁹ Francoise Mascart ^{10, 5} Jose G Ruiz de Morales ¹¹ Catherine Ottone ^{6, 2} Lydia Redjah ⁵ Nina Salabert-Le Guen ^{3, 4} Alain Savenay ¹² Manfred Schmolz ¹³ Antoine Toubert ¹² Matthew L Albert ^{1, 2, 14}

1 Immunobiologie des Cellules dendritiques

2 CRT - Centre de Recherche Translationnelle

3 Laboratoire d’Immunologie, Centre d’Immunomonitorage Nantes Atlantique (CIMNA)

4 U1064 Inserm - CRTI - Centre de Recherche en Transplantation et Immunologie

5 laboratoire de vaccinologie et immunité mucosale

6 ICAReB - Investigation Clinique et d’Accès aux Ressources Biologiques (Plate-forme) - Clinical Investigation and Access to BioResources

7 Myriad RBM

8 U932 - Immunité et cancer

9 Hospital Universitari Germans Trias I Pujol

10 Immunobiology Clinic

11 Complejo Asistencial Universitario de León

12 A2T - Alloimmunité-Autoimmunité-Transplantation

13 HOT Screen GmbH

14 Genentech, Inc. [San Francisco]

Abstract : Functional immune responses are increasingly important for clinical studies, providing in depth biomarker information to assess immunotherapy or vaccination. Incorporating functional immune assays into routine clinical practice has remained limited due to challenges in standardizing sample preparation. We recently described the use of a whole blood syringe-based system, TruCulture®, which permits point-of-care standardized immune stimulation. Here, we report on a multi-center clinical study in seven FOCIS Centers of Excellence to directly compare TruCulture to conventional PBMC methods. Whole blood and PBMCs from healthy donors were exposed to LPS, anti-CD3 anti-CD28 antibodies, or media alone. 55 protein analytes were analyzed centrally by Luminex multi-analyte profiling in a CLIA-certified laboratory. TruCulture responses showed greater reproducibility and improved the statistical power for monitoring differential immune response activation. The use of TruCulture addresses a major unmet need through a robust and flexible method for immunomonitoring that can be reproducibly applied in multi-center clinical studies.

Keywords : PBMCs Whole blood Multi-center clinical studies Cytokines Functional immune responses Immunomonitoring

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Immunology

Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01620308
Contributor : Marie-Christine Vougny <>
Submitted on : Friday, October 20, 2017 - 1:21:01 PM
Last modification on : Saturday, May 18, 2019 - 6:24:01 PM

Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

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Standardized whole blood stimulation improves immunomonitoring of induced immune responses in multi-center study

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