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Guidelines for the use of flow cytometry and cell sorting in immunological studies

Andrea Cossarizza ¹ Hyun-Dong Chang ² Andreas Radbruch ³ Immanuel Andrä Francesco Annunziato ⁴ Petra Bacher Regina Barnaba Luca Battistini ⁵ Wolfgang Bauer ⁶ Sabine Baumgart ⁷ Burkhard Becher Wolfgang Beisker ⁸ Claudia Berek ⁹ Alfonso Blanco Giovanna Borsellino ¹⁰ David Boulais Ryan R Brinkman ¹¹ Martin Büscher Dirk Busch Timothy P Bushnell Xuetao Cao ¹² Andrea Cavani Pratip K Chattopadhyay Qingyu Cheng Sue Chow Mario Clerici ¹³ Anne Cooke Antonio Cosma ¹⁴ Lorenzo Cosmi van Duc Dang Derek Davies Mor de Biasi Genny del Zotto Silvia Della Bella Paolo Dellabona ¹⁵ Günnur Deniz Mark Dessing Andreas Diefenbach James P. Di Santo ¹⁶ Francesco Dieli Bo Dolf Vera S Donnenberg Thomas Dörner ¹⁷ Götz R A Ehrhardt Bo Endl Pablo Engel Britta Engelhardt Charlotte Esser Bart Everts Christine Falk Todd A Fehniger Andrew Filby Simon Simon Marie Follo Bo Förster John Foster John Foulds Paul S Frenette Ari Galbraith Bo Garbi Maria Dolores García-Godoy Kamran Ghoreschi Mor Gibellini Christoph Goettlinger Carl S Goodyear Andrea Gori ¹⁸ Francisco Grogan Mor Gross Andreas Grützkau ¹⁹ Daryl Grummitt Alexander Hahn Quirin Hammer Anja E Hauser David Houston David Hedley Guadalupe Herrera Alexander Herrmann ²⁰ Falk Hiepe Bo Holland Pleun Hombrink Jessica P Houston Bimba F Hoyer Ton Huang Christopher A Hunter Anna Iannone Hans-Martin Jäck Beatriz Jávega Stipan Jonjic ²¹ Kerstin Juelke Steffen Jung ²² Toralf Kaiser Tomas Kalina Baerbel Keller Srijit Khan Alexander Kienhöfer Thomas Kroneis Désirée Kunkel ²³ Christian Kurts ²⁴ Pia Kvistborg Virginia Lannigan Olivier Lantz ²⁵ Anis A Larbi ²⁶ Salomé Leibundgut-Landmann ²⁷ Michael D Leipold Megan K Levings Virginia Litwin Yanling Liu ²⁸ Michael Lohoff ²⁹ Giovanna Lombardi ³⁰ Lilly Lopez Amy Lovett-Racke Erik Lubberts ³¹ Burkhard Ludewig Enrico Lugli ³² Holden T Maecker Gloria Martrus ³³ Giuseppe Matarese Alexander Maueröder Mairi Mcgrath ³⁴ Iain Mcinnes Henrik E Mei Fritz Melchers Susanne Melzer Dirk Mielenz Kingston Mills Jenny Mjösberg ³⁵ Jonni Moore Barry Moran Alessandro Moretta ³⁶ Lorenzo Moretta ³⁶ David Mosmann Susann Müller ³⁷ Werner Müller ³⁸ Christian Münz ³⁹ Gabriele Multhoff ⁴⁰ Alexander Munoz Kenneth M Murphy Toshinori Nakayama Milena Nasi Christine Neudörfl John Nolan ⁴¹ Sussan Nourshargh ⁴² José-Enrique O'Connor Francisco Ouyang Annette Oxenius Raghav Palankar Isabel Panse ² Peter Peterson Christian Peth Jordi Petriz Daisy Philips Winfried Pickl Regina Piconese Marcello Pinti a Graham Pockley Alexander Podolska Carlo Pucillo David Quataert Timothy R D J Radstake Bartek Rajwa ⁴³ David Rebhahn Diether Recktenwald Ester B M Remmerswaal Katy Rezvani Laura G Rico J Paul Robinson Chiara Romagnani Anna Rubartelli Jürgen Ruland Shimon Sakaguchi ⁴⁴ Francisco Sala-De-Oyanguren Yvonne Samstag Sharon Sanderson Birgit Sawitzki Alexander Scheffold Matthias Schiemann Frank Schildberg Esther Schimisky Stephan A Schmid Steffen Schmitt ⁴⁵ Kilian Schober Thomas Schüler ⁴⁶ Axel Ronald Schulz Ton Schumacher Cristiano Scotta T Vincent Shankey Anat Shemer Anna-Katharina Simon Josef Spidlen ¹¹ Alan M Stall Regina Stark Christina Stehle Merle Stein Tobit Steinmetz Hannes Stockinger ⁴⁷ Yousuke Takahama Attila Tarnok ⁴⁸ Zhigang Tian ⁴⁹ Gergely Toldi Julia Tornack Elisabetta Traggiai Joe Trotter Paulo Ulrich Marlous van Der Braber René a W van Lier Marcello Veldhoen Salvador Vento-Asturias Paulo Vieira ⁵⁰ David Voehringer ⁵¹ Hans-Dieter Volk ³ Konrad von Volkmann Ari Waisman ⁵² Rachael Walker Michael D Ward Klaus Warnatz Sarah Warth James Watson Carsten Watzl Leonie Wegener Annika Wiedemann Jürgen Wienands Gerald Willimsky James Wing Peter Wurst Liping Yu Alice Yue Qianjun Zhang Yi Zhao ⁵³ Susanne Ziegler ⁵⁴ Jakob Zimmermann

1 Dipartimento di Scienze Biomediche

2 Cell Biology

3 Klinik für Dermatologie, Venerologie und Allergologie

4 Department of Internal Medicine

5 Neuroimmunology Unit

6 Inorganic Chemistry II

7 Caprotec Bioanalytics GmbH

8 IOTA ES - International Occultation Timing Association European Section

9 Institut der Leibniz-Gemeinschaft, Berlin

10 IRCCS - Fondazione Santa Lucia

11 Terry Fox Laboratory

12 Department of Immunology

13 Fondazione Don Carlo Gnocchi

14 IMVA - U1184 - Immunologie des Maladies Virales et Autoimmunes

15 Experimental Immunology Unit, Dept. of Oncology

16 Département d'Immunologie - Department of Immunology

17 Charité Hospital

18 UB - Universitat de Barcelona

19 Rheumatologie

20 Department of Internal Medicine I

21 Department of Histology and Embryology

22 Weizmann Institute of Science [Rehovot, Israël]

23 Régulation des Infections Rétrovirales

24 Institutes of Molecular Medicine and Experimental Immunology

25 U932 - Immunité et cancer

26 SIgN - Singapore Immunology Network

27 Institute of Virology [Zürich]

28 College of Food Science and Technology [Shangai]

29 Institute for Medical Microbiology and Hygiene

30 MRC - Centre for Transplantation, King's College London

31 Erasmus MC - Erasmus University Medical Center [Rotterdam]

32 Unit of Clinical and Experimental Immunology

33 Heinrich Pette Institute [Hamburg]

34 Institute of Translational Medicine

35 Department of Medicine Huddinge

36 UniGe - Università degli studi di Genova = University of Genoa

37 Department of Environmental Microbiology

38 Experimental Immunology

39 Viral Immunobiology

40 Department of Radiation Oncology [Munich]

41 Department of Mathematics and Statistics

42 William Harvey Research Institute, Barts and the London Medical School

43 Cytometry Laboratories and School of Veterinary Medicine

44 Osaka University [Osaka]

45 FACS and Array Core Facility

46 Institute for Cognitive Science

47 Department of Molecular Immunology

48 Universität Leipzig [Leipzig]

49 Institute of Immunology

50 Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4) - Lymphopoïèse

51 Institute for Immunology

52 Department of Internal Medicine

53 PROTEE - Processus de Transfert et d'Echanges dans l'Environnement - EA 3819

54 Heinrich-Pette-Institut

Abstract : The classical model of hematopoiesis established in the mouse postulates that lymphoid cells originate from a founder population of common lymphoid progenitors. Here, using a modeling approach in humanized mice, we showed that human lymphoid development stemmed from distinct populations of CD127(-) and CD127(+) early lymphoid progenitors (ELPs). Combining molecular analyses with in vitro and in vivo functional assays, we demonstrated that CD127(-) and CD127(+) ELPs emerged independently from lympho-mono-dendritic progenitors, responded differently to Notch1 signals, underwent divergent modes of lineage restriction, and displayed both common and specific differentiation potentials. Whereas CD127(-) ELPs comprised precursors of T cells, marginal zone B cells, and natural killer (NK) and innate lymphoid cells (ILCs), CD127(+) ELPs supported production of all NK cell, ILC, and B cell populations but lacked T potential. On the basis of these results, we propose a "two-family" model of human lymphoid development that differs from the prevailing model of hematopoiesis.

Keywords : natural killer and innate lymphoid cells differentiation early lymphoid progenitors B cell ontogeny Human lymphopoiesis T cell precursors

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Immunology

Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-01619848
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Submitted on : Thursday, October 19, 2017 - 5:11:07 PM
Last modification on : Monday, November 28, 2022 - 5:32:06 PM