Journal articles

Guidelines for the use of flow cytometry and cell sorting in immunological studies

Andrea Cossarizza ¹ Hyun-Dong Chang ² Andreas Radbruch ³ Immanuel Andrä Francesco Annunziato ⁴ Petra Bacher Regina Barnaba Luca Battistini ⁵ Wolfgang Bauer ⁶ Sabine Baumgart ⁷ Burkhard Becher Wolfgang Beisker ⁸ Claudia Berek ⁹ Alfonso Blanco Giovanna Borsellino ¹⁰ David Boulais Ryan R Brinkman ¹¹ Martin Büscher Dirk Busch Timothy P Bushnell Xuetao Cao ¹² Andrea Cavani Pratip K Chattopadhyay Qingyu Cheng Sue Chow Mario Clerici ¹³ Anne Cooke Antonio Cosma ¹⁴ Lorenzo Cosmi van Duc Dang Derek Davies Mor de Biasi Genny del Zotto Silvia Della Bella Paolo Dellabona ¹⁵ Günnur Deniz Mark Dessing Andreas Diefenbach James P. Di Santo ¹⁶ Francesco Dieli Bo Dolf Vera S Donnenberg Thomas Dörner ¹⁷ Götz R A Ehrhardt Bo Endl Pablo Engel Britta Engelhardt Charlotte Esser Bart Everts Christine Falk Todd A Fehniger Andrew Filby Simon Simon Marie Follo Bo Förster John Foster John Foulds Paul S Frenette Ari Galbraith Bo Garbi Maria Dolores García-Godoy Kamran Ghoreschi Mor Gibellini Christoph Goettlinger Carl S Goodyear Andrea Gori ¹⁸ Francisco Grogan Mor Gross Andreas Grützkau ¹⁹ Daryl Grummitt Alexander Hahn Quirin Hammer Anja E Hauser David Houston David Hedley Guadalupe Herrera Alexander Herrmann ²⁰ Falk Hiepe Bo Holland Pleun Hombrink Jessica P Houston Bimba F Hoyer Ton Huang Christopher A Hunter Anna Iannone Hans-Martin Jäck Beatriz Jávega Stipan Jonjic ²¹ Kerstin Juelke Steffen Jung ²² Toralf Kaiser Tomas Kalina Baerbel Keller Srijit Khan Alexander Kienhöfer Thomas Kroneis Désirée Kunkel ²³ Christian Kurts ²⁴ Pia Kvistborg Virginia Lannigan Olivier Lantz ²⁵ Anis A Larbi ²⁶ Salomé Leibundgut-Landmann ²⁷ Michael D Leipold Megan K Levings Virginia Litwin yanling Liu ²⁸ Michael Lohoff ²⁹ Giovanna Lombardi ³⁰ Lilly Lopez Amy Lovett-Racke Erik Lubberts ³¹ Burkhard Ludewig Enrico Lugli ³² Holden T Maecker Gloria Martrus ³³ Giuseppe Matarese Alexander Maueröder Mairi Mcgrath ³⁴ Iain Mcinnes Henrik E Mei Fritz Melchers Susanne Melzer Dirk Mielenz Kingston Mills Jenny Mjösberg ³⁵ Jonni Moore Barry Moran Alessandro Moretta ³⁶ Lorenzo Moretta ³⁶ David Mosmann Susann Müller ³⁷ Werner Müller ³⁸ Christian Münz ³⁹ Gabriele Multhoff ⁴⁰ Alexander Munoz Kenneth M Murphy Toshinori Nakayama Milena Nasi Christine Neudörfl John Nolan ⁴¹ Sussan Nourshargh ⁴² José-Enrique O'Connor Francisco Ouyang Annette Oxenius Raghav Palankar Isabel Panse ² Peter Peterson Christian Peth Jordi Petriz Daisy Philips Winfried Pickl Regina Piconese Marcello Pinti a Graham Pockley Alexander Podolska Carlo Pucillo David Quataert Timothy R D J Radstake Bartek Rajwa ⁴³ David Rebhahn Diether Recktenwald Ester B M Remmerswaal Katy Rezvani Laura G Rico J Paul Robinson Chiara Romagnani Anna Rubartelli Jürgen Ruland Shimon Sakaguchi ⁴⁴ Francisco Sala-De-Oyanguren yvonne Samstag Sharon Sanderson Birgit Sawitzki Alexander Scheffold Matthias Schiemann Frank Schildberg Esther Schimisky Stephan A Schmid Steffen Schmitt ⁴⁵ Kilian Schober Thomas Schüler ⁴⁶ Axel Ronald Schulz Ton Schumacher Cristiano Scotta T Vincent Shankey Anat Shemer Anna-Katharina Simon Josef Spidlen ¹¹ Alan M Stall Regina Stark Christina Stehle Merle Stein Tobit Steinmetz Hannes Stockinger ⁴⁷ yousuke Takahama Attila Tarnok ⁴⁸ Zhigang Tian ⁴⁹ Gergely Toldi Julia Tornack Elisabetta Traggiai Joe Trotter Paulo Ulrich Marlous van Der Braber René a W van Lier Marcello Veldhoen Salvador Vento-Asturias Paulo Vieira ⁵⁰ David Voehringer ⁵¹ Hans-Dieter Volk ³ Konrad von Volkmann Ari Waisman ⁵² Rachael Walker Michael D Ward Klaus Warnatz Sarah Warth James Watson Carsten Watzl Leonie Wegener Annika Wiedemann Jürgen Wienands Gerald Willimsky James Wing Peter Wurst Liping yu Alice yue Qianjun Zhang yi Zhao ⁵³ Susanne Ziegler ⁵⁴ Jakob Zimmermann

1 Dipartimento di Scienze Biomediche

2 Cell Biology

3 Klinik für Dermatologie, Venerologie und Allergologie

4 Department of Internal Medicine

5 Neuroimmunology Unit

6 Inorganic Chemistry II

7 Caprotec Bioanalytics GmbH

8 IOTA ES - International Occultation Timing Association European Section

9 Institut der Leibniz-Gemeinschaft, Berlin

10 IRCCS - Fondazione Santa Lucia

11 Terry Fox Laboratory

12 Department of Immunology

13 Fondazione Don Carlo Gnocchi

14 IMVA - U1184 - Immunologie des Maladies Virales et Autoimmunes

15 Experimental Immunology Unit, Dept. of Oncology

16 Département d'Immunologie - Department of Immunology

17 Charité Hospital

18 UB - Universitat de Barcelona

19 Rheumatologie

20 Department of Internal Medicine I

21 Department of Histology and Embryology

22 Weizmann Institute of Science [Rehovot, Israël]

23 Régulation des Infections Rétrovirales

24 Institutes of Molecular Medicine and Experimental Immunology

25 U932 - Immunité et cancer

26 SIgN - Singapore Immunology Network

27 Institute of Virology [Zürich]

28 College of Food Science and Technology [Shangai]

29 Institute for Medical Microbiology and Hygiene

30 MRC - Centre for Transplantation, King's College London

31 Erasmus MC - Erasmus University Medical Center [Rotterdam]

32 Unit of Clinical and Experimental Immunology

33 Heinrich Pette Institute [Hamburg]

34 Institute of Translational Medicine

35 Department of Medicine Huddinge

36 Universita degli studi di Genova

37 Department of Environmental Microbiology

38 Experimental Immunology

39 Viral Immunobiology

40 Department of Radiation Oncology [Munich]

41 Department of Mathematics and Statistics

42 William Harvey Research Institute, Barts and the London Medical School

43 Cytometry Laboratories and School of Veterinary Medicine

44 Osaka University [Osaka]

45 FACS and Array Core Facility

46 Institute for Cognitive Science

47 Department of Molecular Immunology

48 Universität Leipzig [Leipzig]

49 Institute of Immunology

50 Lymphopoïèse (UMR_1223 / U1223 / U-Pasteur_4) - Lymphopoïèse

51 Institute for Immunology

52 Department of Internal Medicine

53 PROTEE - Processus de Transfert et d'Echanges dans l'Environnement - EA 3819

54 Heinrich-Pette-Institut

Abstract : The classical model of hematopoiesis established in the mouse postulates that lymphoid cells originate from a founder population of common lymphoid progenitors. Here, using a modeling approach in humanized mice, we showed that human lymphoid development stemmed from distinct populations of CD127(-) and CD127(+) early lymphoid progenitors (ELPs). Combining molecular analyses with in vitro and in vivo functional assays, we demonstrated that CD127(-) and CD127(+) ELPs emerged independently from lympho-mono-dendritic progenitors, responded differently to Notch1 signals, underwent divergent modes of lineage restriction, and displayed both common and specific differentiation potentials. Whereas CD127(-) ELPs comprised precursors of T cells, marginal zone B cells, and natural killer (NK) and innate lymphoid cells (ILCs), CD127(+) ELPs supported production of all NK cell, ILC, and B cell populations but lacked T potential. On the basis of these results, we propose a "two-family" model of human lymphoid development that differs from the prevailing model of hematopoiesis.

Keywords : natural killer and innate lymphoid cells differentiation early lymphoid progenitors B cell ontogeny Human lymphopoiesis T cell precursors

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Immunology

Complete list of metadata

https://hal-pasteur.archives-ouvertes.fr/pasteur-01619848
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Submitted on : Thursday, October 19, 2017 - 5:11:07 PM
Last modification on : Saturday, June 25, 2022 - 9:10:52 PM