Guidelines for the use of flow cytometry and cell sorting in immunological studies

Andrea Cossarizza 1 Hyun-Dong Chang 2 Andreas Radbruch 3 Immanuel Andrä Francesco Annunziato 4 Petra Bacher Regina Barnaba Luca Battistini 5 Wolfgang Bauer 6 Sabine Baumgart 7 Burkhard Becher Wolfgang Beisker 8 Claudia Berek 9 Alfonso Blanco Giovanna Borsellino 10 David Boulais Ryan R Brinkman 11 Martin Büscher Dirk Busch Timothy P Bushnell Xuetao Cao 12 Andrea Cavani Pratip K Chattopadhyay Qingyu Cheng Sue Chow Mario Clerici 13 Anne Cooke Antonio Cosma 14 Lorenzo Cosmi Van Duc Dang Derek Davies Mor De Biasi Genny Del Zotto Silvia Della Bella Paolo Dellabona 15 Günnur Deniz Mark Dessing Andreas Diefenbach James Di Santo 16 Francesco Dieli Bo Dolf Vera S Donnenberg Thomas Dörner 17 Götz R A Ehrhardt Bo Endl Pablo Engel Britta Engelhardt Charlotte Esser Bart Everts Christine Falk Todd A Fehniger Andrew Filby Simon Simon Marie Follo Bo Förster John Foster John Foulds Paul S Frenette Ari Galbraith Bo Garbi Maria Dolores García-Godoy Kamran Ghoreschi Mor Gibellini Christoph Goettlinger Carl S Goodyear Andrea Gori 18 Francisco Grogan Mor Gross Andreas Grützkau 19 Daryl Grummitt Alexander Hahn Quirin Hammer Anja E Hauser David Houston David Hedley Guadalupe Herrera Alexander Herrmann 20 Falk Hiepe Bo Holland Pleun Hombrink Jessica P Houston Bimba F Hoyer Ton Huang Christopher A Hunter Anna Iannone Hans-Martin Jäck Beatriz Jávega Stipan Jonjic 21 Kerstin Juelke Steffen Jung 22 Toralf Kaiser Tomas Kalina Baerbel Keller Srijit Khan Alexander Kienhöfer Thomas Kroneis Désirée Kunkel 23 Christian Kurts 24 Pia Kvistborg Virginia Lannigan Olivier Lantz 25 Anis Larbi 26 Salomé Leibundgut-Landmann 27 Michael D Leipold Megan K Levings Virginia Litwin Yanling Liu 28 Michael Lohoff 29 Giovanna Lombardi 30 Lilly Lopez Amy Lovett-Racke Erik Lubberts 31 Burkhard Ludewig Enrico Lugli 32 Holden T Maecker Gloria Martrus 33 Giuseppe Matarese Alexander Maueröder Mairi Mcgrath 34 Iain Mcinnes Henrik E Mei Fritz Melchers Susanne Melzer Dirk Mielenz Kingston Mills Jenny Mjösberg 35 Jonni Moore Barry Moran Alessandro Moretta 36 Lorenzo Moretta 36 David Mosmann Susann Müller 37 Werner Müller 38 Christian Münz 39 Gabriele Multhoff 40 Alexander Munoz Kenneth M Murphy Toshinori Nakayama Milena Nasi Christine Neudörfl John Nolan 41 Sussan Nourshargh 42 José-Enrique O'Connor Francisco Ouyang Annette Oxenius Raghav Palankar Isabel Panse 2 Peter Peterson Christian Peth Jordi Petriz Daisy Philips Winfried Pickl Regina Piconese Marcello Pinti A Graham Pockley Alexander Podolska Carlo Pucillo David Quataert Timothy R D J Radstake Bartek Rajwa 43 David Rebhahn Diether Recktenwald Ester B M Remmerswaal Katy Rezvani Laura G Rico J Paul Robinson Chiara Romagnani Anna Rubartelli Jürgen Ruland Shimon Sakaguchi 44 Francisco Sala-De-Oyanguren Yvonne Samstag Sharon Sanderson Birgit Sawitzki Alexander Scheffold Matthias Schiemann Frank Schildberg Esther Schimisky Stephan A Schmid Steffen Schmitt 45 Kilian Schober Thomas Schüler 46 Axel Ronald Schulz Ton Schumacher Cristiano Scotta T Vincent Shankey Anat Shemer Anna-Katharina Simon Josef Spidlen 11 Alan M Stall Regina Stark Christina Stehle Merle Stein Tobit Steinmetz Hannes Stockinger 47 Yousuke Takahama Attila Tarnok 48 Zhigang Tian 49 Gergely Toldi Julia Tornack Elisabetta Traggiai Joe Trotter Paulo Ulrich Marlous Van Der Braber René a W Van Lier Marcello Veldhoen Salvador Vento-Asturias Paulo Vieira 50 David Voehringer 51 Hans-Dieter Volk 3 Konrad Von Volkmann Ari Waisman 52 Rachael Walker Michael D Ward Klaus Warnatz Sarah Warth James Watson Carsten Watzl Leonie Wegener Annika Wiedemann Jürgen Wienands Gerald Willimsky James Wing Peter Wurst Liping Yu Alice Yue Qianjun Zhang Yi Zhao 53 Susanne Ziegler 54 Jakob Zimmermann
Abstract : The classical model of hematopoiesis established in the mouse postulates that lymphoid cells originate from a founder population of common lymphoid progenitors. Here, using a modeling approach in humanized mice, we showed that human lymphoid development stemmed from distinct populations of CD127(-) and CD127(+) early lymphoid progenitors (ELPs). Combining molecular analyses with in vitro and in vivo functional assays, we demonstrated that CD127(-) and CD127(+) ELPs emerged independently from lympho-mono-dendritic progenitors, responded differently to Notch1 signals, underwent divergent modes of lineage restriction, and displayed both common and specific differentiation potentials. Whereas CD127(-) ELPs comprised precursors of T cells, marginal zone B cells, and natural killer (NK) and innate lymphoid cells (ILCs), CD127(+) ELPs supported production of all NK cell, ILC, and B cell populations but lacked T potential. On the basis of these results, we propose a "two-family" model of human lymphoid development that differs from the prevailing model of hematopoiesis.
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European Journal of Immunology, Wiley-VCH Verlag, 2017, 47 (10), pp.1584 - 1797. 〈10.1002/eji.201646632〉
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Soumis le : jeudi 19 octobre 2017 - 17:11:07
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Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Immanuel Andrä, Francesco Annunziato, et al.. Guidelines for the use of flow cytometry and cell sorting in immunological studies. European Journal of Immunology, Wiley-VCH Verlag, 2017, 47 (10), pp.1584 - 1797. 〈10.1002/eji.201646632〉. 〈pasteur-01619848〉

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