Abstract : Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.
https://hal-pasteur.archives-ouvertes.fr/pasteur-01574133
Contributor : Marie-Therese Vicente
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Submitted on : Friday, August 11, 2017 - 4:08:05 PM
Last modification on : Tuesday, October 23, 2018 - 11:46:02 AM
Juan J Quereda, Martin Sachse, Damien Balestrino, Théodore Grenier, Jennifer Fredlund, et al.. Assessing Vacuolar Escape of Listeria Monocytogenes.. Collin M. Bacterial Pathogenesis, 1535, Humana Press, New York, NY, pp.173-195, 2016, Methods in Molecular Biology, 978-1-4939-6671-4. ⟨10.1007/978-1-4939-6673-8_11⟩. ⟨pasteur-01574133⟩
