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Assessing Vacuolar Escape of Listeria Monocytogenes.

Juan J Quereda 1 Martin Sachse 2 Damien Balestrino 1, 3 Théodore Grenier 1 Jennifer Fredlund 4 Anne Danckaert 5 Nathalie Aulner 5 Spencer Shorte 5 Jost Enninga 4 Pascale Cossart 1 Javier Pizarro-Cerdá 1, *
* Corresponding author
1 UIBC - Interactions Bactéries-Cellules
2 CITECH - Microscopie ultrastructurale - Ultrapole
3 LMGE - Laboratoire Microorganismes : Génome et Environnement
4 Dynamique des Interactions Hôte-Pathogène - Dynamics of Host-Pathogen Interactions
5 CITECH - Imagopole
Abstract : Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.
Keywords : Vacuole Phospholipases Phagocytosis Listeriolysin O Listeria monocytogenes Förster resonance energy transfer (FRET) microscopy CCF4 Correlative light/electron microcopy (CLEM)
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Juan J Quereda, Martin Sachse, Damien Balestrino, Théodore Grenier, Jennifer Fredlund, et al.. Assessing Vacuolar Escape of Listeria Monocytogenes.. Collin M. Bacterial Pathogenesis, 1535, Humana Press, New York, NY, pp.173-195, 2016, Methods in Molecular Biology, 978-1-4939-6671-4. ⟨10.1007/978-1-4939-6673-8_11⟩. ⟨pasteur-01574133⟩

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