Assessing Vacuolar Escape of Listeria Monocytogenes.

Juan J Quereda (1) , Martin Sachse (2) , Damien Balestrino (1, 3) , Théodore Grenier (1) , Jennifer Fredlund (4) , Anne Danckaert (5) , Nathalie Aulner (5) , Spencer Shorte (5) , Jost Enninga (4) , Pascale Cossart (1) , Javier Pizarro-Cerdá (1)

1 UIBC - Interactions Bactéries-Cellules
2 CITECH - Microscopie ultrastructurale - Ultrapole
3 LMGE - Laboratoire Microorganismes : Génome et Environnement
4 Dynamique des Interactions Hôte-Pathogène - Dynamics of Host-Pathogen Interactions
5 CITECH - Imagopole

Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.

Keywords: Listeria monocytogenes; Vacuole; Phospholipases; Phagocytosis; Listeriolysin O; Förster resonance energy transfer (FRET) microscopy; CCF4; Correlative light/electron microcopy (CLEM)

Domains: Life Sciences [q-bio]; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Juan J Quereda, Martin Sachse, Damien Balestrino, Théodore Grenier, Jennifer Fredlund, et al.. Assessing Vacuolar Escape of Listeria Monocytogenes.. Collin M. Bacterial Pathogenesis, 1535, Humana Press, New York, NY, pp.173-195, 2016, Methods in Molecular Biology, 978-1-4939-6671-4. ⟨10.1007/978-1-4939-6673-8_11⟩. ⟨pasteur-01574133⟩