Assessing Vacuolar Escape of Listeria Monocytogenes.

Juan J Quereda; Martin Sachse; Damien Balestrino; Théodore Grenier; Jennifer Fredlund; Anne Danckaert; Nathalie Aulner; Spencer Shorte; Jost Enninga; Pascale Cossart; Javier Pizarro-Cerdá

doi:10.1007/978-1-4939-6673-8_11

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Assessing Vacuolar Escape of Listeria Monocytogenes.

Juan J Quereda ¹ Martin Sachse ² Damien Balestrino ^{1, 3} Théodore Grenier ¹ Jennifer Fredlund ⁴ Anne Danckaert ⁵ Nathalie Aulner ⁵ Spencer Shorte ⁵ Jost Enninga ⁴ Pascale Cossart ¹ Javier Pizarro-Cerdá ^{1, *}

* Corresponding author

1 UIBC - Interactions Bactéries-Cellules

2 CITECH - Microscopie ultrastructurale - Ultrapole

3 LMGE - Laboratoire Microorganismes : Génome et Environnement

4 Dynamique des Interactions Hôte-Pathogène - Dynamics of Host-Pathogen Interactions

5 CITECH - Imagopole

Abstract : Listeria monocytogenes is a bacterial pathogen which invades and multiplies within non-professional phagocytes. Signaling cascades involved in cellular entry have been extensively analyzed, but the events leading to vacuolar escape remain less clear. In this chapter, we detail a microscopy FRET-based assay which allows quantitatively measuring L. monocytogenes infection and escape from its internalization vacuole, as well as a correlative light/electron microscopy method to investigate the morphological features of the vacuolar compartments containing L. monocytogenes.

Keywords : Vacuole Phospholipases Phagocytosis Listeriolysin O Listeria monocytogenes Förster resonance energy transfer (FRET) microscopy CCF4 Correlative light/electron microcopy (CLEM)

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Life Sciences [q-bio]

Life Sciences [q-bio] / Microbiology and Parasitology / Bacteriology

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