Alteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins.

Abstract : Bacterial pathogens can interfere during infection with host cell organelles, such as mitochondria, the endoplasmic reticulum-Golgi system or nuclei. As important cellular functions are often compartmentalized in these organelles, their targeting allows pathogens to manipulate key host functions during infection. Here, we identify lysosomes as a new class of organelles targeted by the pathogenic bacterium Listeria monocytogenes. We demonstrate that extracellular Listeria, via secretion of the pore-forming toxin listeriolysin O, alters lysosomal integrity in epithelial cells but not in macrophages. Listeriolysin O induces lysosomal membrane permeabilization and release of lysosomal content, such as cathepsins proteases, which remain transiently active in the host cytosol. We furthermore show that other bacterial pore-forming toxins, such as perfringolysin O and pneumolysin, also induce lysosomes alteration. Together, our data unveil a novel activity of bacterial cholesterol-dependent cytolysins.
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Julien Karim Malet, Pascale Cossart, David Ribet. Alteration of epithelial cell lysosomal integrity induced by bacterial cholesterol-dependent cytolysins.. Cellular Microbiology, Wiley, 2017, 19 (4), pp.e12682 ⟨https://doi.org/10.1111/cmi.12682⟩. ⟨10.1111/cmi.12682⟩. ⟨pasteur-01574071⟩

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