Comparative genomic analysis of Acinetobacter strains isolated from murine colonic crypts

Abstract : Background: A restricted set of aerobic bacteria dominated by the Acinetobacter genus was identified in murine intestinal colonic crypts. The vicinity of such bacteria with intestinal stem cells could indicate that they protect the crypt against cytotoxic and genotoxic signals. Genome analyses of these bacteria were performed to better appreciate their biodegradative capacities. Results: Two taxonomically different clusters of Acinetobacter were isolated from murine proximal colonic crypts, one was identified as A. modestus and the other as A. radioresistens. Their identification was performed through biochemical parameters and housekeeping gene sequencing. After selection of one strain of each cluster (A. modestus CM11G and A. radioresistens CM38.2), comparative genomic analysis was performed on whole-genome sequencing data. The antibiotic resistance pattern of these two strains is different, in line with the many genes involved in resistance to heavy metals identified in both genomes. Moreover whereas the operon benABCDE involved in benzoate metabolism is encoded by the two genomes, the operon antABC encoding the anthranilate dioxygenase, and the phenol hydroxylase gene cluster are absent in the A. modestus genomic sequence, indicating that the two strains have different capacities to metabolize xenobiotics. A common feature of the two strains is the presence of a type IV pili system, and the presence of genes encoding proteins pertaining to secretion systems such as Type I and Type II secretion systems. Conclusions: Our comparative genomic analysis revealed that different Acinetobacter isolated from the same biological niche, even if they share a large majority of genes, possess unique features that could play a specific role in the protection of the intestinal crypt.
Document type :
Journal articles
Complete list of metadatas

Cited literature [49 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01570221
Contributor : Floran Bidault <>
Submitted on : Friday, July 28, 2017 - 4:20:59 PM
Last modification on : Thursday, January 11, 2018 - 6:27:26 AM

File

document.pdf
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Azadeh Saffarian, Marie Touchon, Céline Mulet, Régis Tournebize, Virginie Passet, et al.. Comparative genomic analysis of Acinetobacter strains isolated from murine colonic crypts. BMC Genomics, BioMed Central, 2017, 18 (1), pp.525. ⟨10.1186/s12864-017-3925-x⟩. ⟨pasteur-01570221⟩

Share

Metrics

Record views

67

Files downloads

117