Lokiarchaea are close relatives of Euryarchaeota, not bridging the gap between prokaryotes and eukaryotes

Violette Da Cunha 1, 2 Morgan Gaia 1 Danièle Gadelle 2 Arshan Nasir 3 Patrick Forterre 2, 1, *
* Auteur correspondant
1 BMGE - Biologie Moléculaire du Gène chez les Extrêmophiles
2 I2BC - Institut de Biologie Intégrative de la Cellule
3 CIIT - COMSATS Institute of Information Technology [Islamabad]
Abstract : The eocyte hypothesis, in which Eukarya emerged from within Archaea, has been boosted by the description of a new candidate archaeal phylum, “Lokiarchaeota”, from metagenomic data. Eukarya branch within Lokiarchaeota in a tree reconstructed from the concatenation of 36 universal proteins. However, individual phylogenies revealed that lokiarchaeal proteins sequences have different evolutionary histories. The individual markers phylogenies revealed at least two subsets of proteins, either supporting the Woese or the Eocyte tree of life. Strikingly, removal of a single protein, the elongation factor EF2, is sufficient to break the Eukaryotes-Lokiarchaea affiliation. Our analysis suggests that the three lokiarchaeal EF2 proteins have a chimeric organization that could be due to contamination and/or homologous recombination with patches of eukaryotic sequences. A robust phylogenetic analysis of RNA polymerases with a new dataset indicates that Lokiarchaeota and related phyla of the Asgard superphylum are sister group to Euryarchaeota, not to Eukarya, and supports the monophyly of Archaea with their rooting in the branch leading to Thaumarchaeota. © 2017 Da Cunha et al.
Keywords : Eukaryota Phylogenetic analysis Archaea Sequence alignment RNA polymerase Phylogenetics Archaean biology Protein structure comparison
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PLoS Genetics, Public Library of Science, 2017, 13 (6), pp.e1006810. 〈10.1371/journal.pgen.1006810〉
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PASTEUR | CEA | I2BC | UNIV-PARIS-SACLAY | CEA-DRF | CEA-UPSAY | JOLIOT

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Violette Da Cunha, Morgan Gaia, Danièle Gadelle, Arshan Nasir, Patrick Forterre. Lokiarchaea are close relatives of Euryarchaeota, not bridging the gap between prokaryotes and eukaryotes. PLoS Genetics, Public Library of Science, 2017, 13 (6), pp.e1006810. 〈10.1371/journal.pgen.1006810〉. 〈pasteur-01570207〉

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