Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions.

Raphael Etournay 1 Ingrid Zwaenepoel 1 Isabelle Perfettini 1 Pierre Legrain 2 Christine Petit 1 Aziz El-Amraoui 1, *
* Auteur correspondant
1 Génétique des Déficits Sensoriels
2 SHFJ - Service Hospitalier Frédéric Joliot
Abstract : Defects in myosin VIIa lead to developmental anomalies of the auditory and visual sensory cells. We sought proteins interacting with the myosin VIIa tail by using the yeast two-hybrid system. Here, we report on shroom2, a submembranous PDZ domain-containing protein that is associated with the tight junctions in multiple embryonic and adult epithelia. Shroom2 directly interacts with the C-terminal MyTH4-FERM domain of myosin VIIa and with F-actin. In addition, a shroom2 fragment containing the region of interaction with F-actin was able to protect actin filaments from cytochalasin-D-induced disruption in MDCK cells. Transfection experiments in MDCK and LE (L fibroblasts that express E-cadherin) cells led us to conclude that shroom2 is targeted to the cell-cell junctions in the presence of tight junctions only. In Ca(2+)-switch experiments on MDCK cells, ZO-1 (also known as TJP1) preceded GFP-tagged shroom2 at the differentiating tight junctions. ZO-1 directly interacts with the serine- and proline-rich region of shroom2 in vitro. Moreover, the two proteins colocalize in vivo at mature tight junctions, and could be coimmunoprecipitated from brain and cochlear extracts. We suggest that shroom2 and ZO-1 form a tight-junction-associated scaffolding complex, possibly linked to myosin VIIa, that bridges the junctional membrane to the underlying cytoskeleton, thereby contributing to the stabilization of these junctions.
Keywords : Myosin VIIa Shroom2 F-actin ZO-1 Tight junctions
Type de document :
Article dans une revue
Journal of Cell Science, Company of Biologists, 2007, 120 (16), pp.2838-50. 〈10.1242/jcs.002568〉
Liste complète des métadonnées

Littérature citée [45 références]  Voir  Masquer  Télécharger
https://hal-pasteur.archives-ouvertes.fr/pasteur-01545829
Contributeur : Raphael Etournay <>
Soumis le : vendredi 23 juin 2017 - 13:07:01
Dernière modification le : mardi 11 décembre 2018 - 01:19:07
Document(s) archivé(s) le : mercredi 10 janvier 2018 - 22:12:48

Fichier

Etournay et al. - 2007 - Shroo...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

PASTEUR | CEA | JOLIOT | CEA-DRF | SHFJ

Citation

Raphael Etournay, Ingrid Zwaenepoel, Isabelle Perfettini, Pierre Legrain, Christine Petit, et al.. Shroom2, a myosin-VIIa- and actin-binding protein, directly interacts with ZO-1 at tight junctions.. Journal of Cell Science, Company of Biologists, 2007, 120 (16), pp.2838-50. 〈10.1242/jcs.002568〉. 〈pasteur-01545829〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

68

Téléchargements de fichiers

157