Type III secretion effectors of the IpaH family are E3 ubiquitin ligases.

Abstract : Many bacteria pathogenic for plants or animals, including Shigella spp., which is responsible for shigellosis in humans, use a type III secretion apparatus to inject effector proteins into host cells. Effectors alter cell signaling and host responses induced upon infection; however, their precise biochemical activities have been elucidated in very few cases. Utilizing Saccharomyces cerevisiae as a surrogate host, we show that the Shigella effector IpaH9.8 interrupts pheromone response signaling by promoting the proteasome-dependent destruction of the MAPKK Ste7. In vitro, IpaH9.8 displayed ubiquitin ligase activity toward ubiquitin and Ste7. Replacement of a Cys residue that is invariant among IpaH homologs of plant and animal pathogens abolished the ubiquitin ligase activity of IpaH9.8. We also present evidence that the IpaH homolog SspH1 from Salmonella enterica can ubiquitinate ubiquitin and PKN1, a previously identified SspH1 interaction partner. This study assigns a function for IpaH family members as E3 ubiquitin ligases.
Type de document :
Article dans une revue
Cell Host and Microbe, Elsevier, 2007, 1 (1), pp.77-83. 〈10.1016/j.chom.2007.02.002〉
Liste complète des métadonnées

Littérature citée [32 références]  Voir  Masquer  Télécharger

Contributeur : Bénédicte Benedic <>
Soumis le : vendredi 16 juin 2017 - 16:43:43
Dernière modification le : vendredi 22 juin 2018 - 13:08:04
Document(s) archivé(s) le : mercredi 10 janvier 2018 - 13:15:20


 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier




John R Rohde, Ashton Breitkreutz, Alexandre Chenal, Philippe J Sansonetti, Claude Parsot. Type III secretion effectors of the IpaH family are E3 ubiquitin ligases.. Cell Host and Microbe, Elsevier, 2007, 1 (1), pp.77-83. 〈10.1016/j.chom.2007.02.002〉. 〈pasteur-01540876〉



Consultations de la notice