Differential expression of Cathepsin E in transthyretin amyloidosis: from neuropathology to the immune system.

Abstract : Background: Increasing evidence supports a key role for inflammation in the neurodegenerative process of familial amyloidotic polyneuropathy (FAP). While there seems to be an overactivation of the neuronal interleukin-1 signaling pathway, the immune response is apparently compromised in FAP. Accordingly, little immune cell infiltration is observed around pre-fibrillar or fibrillar amyloid deposits, with the underlying mechanism for this phenomenon remaining poorly understood. Cathepsin E (CtsE) is an important intermediate for antigen presentation and chemotaxis, but its role in the pathogenesis of FAP disease remains unknown. Methods: In this study, we used both mouse primary macrophages and in vivo studies based on transgenic models of FAP and human samples to characterize CtsE expression in different physiological systems. Results: We show that CtsE is critically decreased in bone marrow-derived macrophages from a FAP mouse model, possibly contributing for cell function impairment. Compromised levels of CtsE were also found in injured nerves of transgenic mice and, most importantly, in naïve peripheral nerves, sensory ganglia, murine stomach, and sural nerve biopsies derived from FAP patients. Expression of CtsE in tissues was associated with transthyretin (TTR) deposition and differentially regulated accordingly with the physiological system under study. Preventing deposition with a TTR small interfering RNA rescued CtsE in the peripheral nervous system (PNS). In contrast, the expression of CtsE increased in splenic cells (mainly monocytes) or peritoneal macrophages, indicating a differential macrophage phenotype. Conclusion: Altogether, our data highlights the potential of CtsE as a novel FAP biomarker and a possible modulator for innate immune cell chemotaxis to the disease most affected tissues-the peripheral nerve and the gastrointestinal tract.
Document type :
Journal articles
Complete list of metadatas

Cited literature [55 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01533696
Contributor : Marie-Christine Vougny <>
Submitted on : Tuesday, June 6, 2017 - 5:12:57 PM
Last modification on : Thursday, April 4, 2019 - 1:26:20 AM

File

art%3A10.1186%2Fs12974-017-089...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Nádia Pereira Gonçalves, João Moreira, Diana Martins, Paulo Vieira, Laura Obici, et al.. Differential expression of Cathepsin E in transthyretin amyloidosis: from neuropathology to the immune system.. Journal of Neuroinflammation, BioMed Central, 2017, 14 (1), pp.115. ⟨10.1186/s12974-017-0891-9⟩. ⟨pasteur-01533696⟩

Share

Metrics

Record views

98

Files downloads

305