19 (d, J ¼ 5.2 Hz95 (s, 3 H, 1-CH 3, 7.94 (d, J ¼ 5.2 Hz, 1 H, 4-H), 7.37 (s, 1 H -H), 0.92 (t, J ¼ 7.4 Hz, 3 H, 4 0 -H). 13 C-NMR (DMSO-D 6 , 400 MHz): d (ppm) ¼ 150, pp.136-143, 2010. ,
The following drugs were used following solubilization in DMSO: tivozanib (Selleck, 43.96 mM stock), cerulenin (TEBU, 50 mM stock), NATURE COMMUNICATIONS | DOI: 10.1038/ncomms11320 ARTICLE Chikungunya virus and the global spread of a mosquito-borne disease. New Engl, NATURE COMMUNICATIONS | J. Med, vol.7, issue.372, pp.1231-1239, 2015. ,
Combating emerging viral threats, Science, vol.98, issue.40, pp.282-283, 2015. ,
DOI : 10.1073/pnas.1009445107
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4419706
The design of drugs for HIV and HCV, Nature Reviews Drug Discovery, vol.64, issue.12, pp.1001-1018, 2007. ,
DOI : 10.1038/nrd2424
Understanding the hepatitis C virus life cycle paves the way for highly effective therapies, Nature Medicine, vol.56, issue.7, pp.837-849, 2013. ,
DOI : 10.1073/pnas.0915117107
Therapeutic Silencing of MicroRNA-122 in Primates with Chronic Hepatitis C Virus Infection, Science, vol.36, issue.4, pp.198-201, 2010. ,
DOI : 10.1093/nar/gkm1113
Curing a viral infection by targeting the host: The example of cyclophilin inhibitors, Antiviral Research, vol.99, issue.1, pp.68-77, 2013. ,
DOI : 10.1016/j.antiviral.2013.03.020
Productive Replication of Ebola Virus Is Regulated by the c-Abl1 Tyrosine Kinase, Science Translational Medicine, vol.20, issue.11, pp.123-124, 2012. ,
DOI : 10.1128/JVI.78.11.5642-5650.2004
Drug repositioning: identifying and developing new uses for existing drugs, Nature Reviews Drug Discovery, vol.2, issue.8, pp.673-683, 2004. ,
DOI : 10.1038/nrd1468
Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication, Nature, vol.4, issue.7282, pp.818-822, 2010. ,
DOI : 10.1038/nature08760
Genome-Wide RNAi Screen Identifies Novel Host Proteins Required for Alphavirus Entry, PLoS Pathogens, vol.55, issue.12, p.1003835, 2013. ,
DOI : 10.1371/journal.ppat.1003835.s011
URL : http://doi.org/10.1371/journal.ppat.1003835
Genome-wide RNAi Screen Identifies SEC61A and VCP as Conserved Regulators of Sindbis Virus Entry, Cell Reports, vol.5, issue.6, pp.1737-1748, 2013. ,
DOI : 10.1016/j.celrep.2013.11.028
URL : http://doi.org/10.1016/j.celrep.2013.11.028
An In??Vivo RNAi Screening Approach to Identify Host Determinants of Virus Replication, Cell Host & Microbe, vol.14, issue.3, pp.346-356, 2013. ,
DOI : 10.1016/j.chom.2013.08.007
Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen, Science, vol.317, issue.5840, pp.921-926, 2008. ,
DOI : 10.1126/science.1143767
Human host factors required for influenza virus replication, Nature, vol.4, issue.7282, pp.813-817, 2010. ,
DOI : 10.1038/nature08699
Analysis of cell-based RNAi screens, Genome Biology, vol.7, issue.7, p.66, 2006. ,
DOI : 10.1186/gb-2006-7-7-r66
A probability-based approach for the analysis of large-scale RNAi screens, Nature Methods, vol.6, issue.10, pp.847-849, 2007. ,
DOI : 10.1038/nmeth1089
cSSMD: assessing collective activity for addressing off-target effects in genome-scale RNA interference screens, Bioinformatics, vol.27, issue.20, pp.2775-2781, 2011. ,
DOI : 10.1093/bioinformatics/btr474
A Functional Genomic Screen Identifies Cellular Cofactors of Hepatitis C Virus Replication, Cell Host & Microbe, vol.5, issue.3, pp.298-307, 2009. ,
DOI : 10.1016/j.chom.2009.02.001
Integrative Functional Genomics of Hepatitis C Virus Infection Identifies Host Dependencies in Complete Viral Replication Cycle, PLoS Pathogens, vol.41, issue.5, p.1004163, 2014. ,
DOI : 10.1371/journal.ppat.1004163.s016
Discovery of insect and human dengue virus host factors, Nature, vol.27, issue.7241, pp.1047-1050, 2009. ,
DOI : 10.1038/nature07967
RNA interference screen for human genes associated with West Nile virus infection, Nature, vol.20, issue.7210, pp.242-245, 2008. ,
DOI : 10.1038/nature07207
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136529
Global Analysis of Host-Pathogen Interactions that Regulate Early-Stage HIV-1 Replication, Cell, vol.135, issue.1, pp.49-60, 2008. ,
DOI : 10.1016/j.cell.2008.07.032
Human genome-wide RNAi screen reveals a role for nuclear pore proteins in poxvirus morphogenesis, Proc. Natl Acad. Sci. USA, pp.3519-3524, 2013. ,
DOI : 10.1006/viro.1993.1424
Dengue virus nonstructural protein 3 redistributes fatty acid synthase to sites of viral replication and increases cellular fatty acid synthesis, Proc. Natl Acad. Sci. USA, pp.17345-17350, 2010. ,
DOI : 10.1186/1475-2875-7-211
Systems-level metabolic flux profiling identifies fatty acid synthesis as a target for antiviral therapy, Nature Biotechnology, vol.80, issue.10, pp.1179-1186, 2008. ,
DOI : 10.1038/nbt.1500
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2825756
Magnetic Fractionation and Proteomic Dissection of Cellular Organelles Occupied by the Late Replication Complexes of Semliki Forest Virus, Journal of Virology, vol.87, issue.18, pp.10295-10312, 2013. ,
DOI : 10.1128/JVI.01105-13
DrugBank 4.0: shedding new light on drug metabolism, Nucleic Acids Research, vol.42, issue.D1, pp.1091-1097, 2014. ,
DOI : 10.1093/nar/gkt1068
Comprehensive analysis of kinase inhibitor selectivity, Nature Biotechnology, vol.114, issue.11, pp.1046-1051, 2011. ,
DOI : 10.1038/nbt.1990
ChEMBL: a large-scale bioactivity database for drug discovery, Nucleic Acids Research, vol.40, issue.D1, pp.1100-1107, 2012. ,
DOI : 10.1093/nar/gkr777
URL : http://doi.org/10.1093/nar/gkr777
Characterization of Reemerging Chikungunya Virus, PLoS Pathogens, vol.4, issue.6, p.89, 2007. ,
DOI : 0889-2229(1988)004[0441:ARASTF]2.0.CO;2
URL : https://hal.archives-ouvertes.fr/pasteur-00161827
Genome-Wide RNAi Screen Identifies Broadly-Acting Host Factors That Inhibit Arbovirus Infection, PLoS Pathogens, vol.412, issue.2 ,
DOI : 10.1371/journal.ppat.1003914.s005
URL : http://doi.org/10.1371/journal.ppat.1003914
A Mouse Model for Chikungunya: Young Age and Inefficient Type-I Interferon Signaling Are Risk Factors for Severe Disease, PLoS Pathogens, vol.41, issue.2, p.29, 2008. ,
DOI : 10.1371/journal.ppat.0040029.sg003
URL : https://hal.archives-ouvertes.fr/pasteur-01402310
Development of Second-Generation VEGFR Tyrosine Kinase Inhibitors: Current Status, Current Oncology Reports, vol.9, issue.2, pp.103-111, 2011. ,
DOI : 10.1007/s11912-011-0154-3
The STAT5 Inhibitor Pimozide Displays Efficacy in Models of Acute Myelogenous Leukemia Driven by FLT3 Mutations, Genes & Cancer, vol.3, issue.7-8, pp.503-511, 2012. ,
DOI : 10.1177/1947601912466555
Antiangiogenic effects of tivozanib, an oral VEGF receptor tyrosine kinase inhibitor, on experimental choroidal neovascularization in mice, vivo imaging of chikungunya virus in mice and Aedes mosquitoes using a Renilla luciferase clone, pp.125-133, 2011. ,
DOI : 10.1016/j.exer.2013.05.006
A Mouse Model of Chikungunya Virus???Induced Musculoskeletal Inflammatory Disease, The American Journal of Pathology, vol.178, issue.1, pp.32-40, 2011. ,
DOI : 10.1016/j.ajpath.2010.11.018
A Pathogenic Role for CD4+ T Cells during Chikungunya Virus Infection in Mice, The Journal of Immunology, vol.190, issue.1, pp.259-269, 2013. ,
DOI : 10.4049/jimmunol.1202177
Development and characterization of a double subgenomic chikungunya virus infectious clone to express heterologous genes in Aedes aegypti mosqutioes, Insect Biochemistry and Molecular Biology, vol.35, issue.10, pp.1162-1170, 2005. ,
DOI : 10.1016/j.ibmb.2005.05.008
Prophylaxis and Therapy for Chikungunya Virus Infection, The Journal of Infectious Diseases, vol.200, issue.4, pp.516-523, 2009. ,
DOI : 10.1086/600381
Virus replicon particle based Chikungunya virus neutralization assay using Gaussia luciferase as readout, Virology Journal, vol.10, issue.1, p.235, 2013. ,
DOI : 10.1371/journal.pone.0028923
URL : https://hal.archives-ouvertes.fr/pasteur-00846927
A DNA transfection system for generation of influenza A virus from eight plasmids, Proc. Natl Acad. Sci. USA 97, pp.6108-6113, 2000. ,
DOI : 10.1073/pnas.96.16.9363
The druggable genome, Nature Reviews Drug Discovery, vol.180, issue.9, pp.727-730, 2002. ,
DOI : 10.1126/science.287.5461.2204
Improved vectors and genome-wide libraries for CRISPR screening, Nature Methods, vol.4, issue.8, pp.783-784, 2014. ,
DOI : 10.1038/nbt.2647
URL : http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4486245/
Bioassay analysis using R, J. Statist. Software, vol.12, pp.1-22, 2005. ,
Synthesis and isolation of bromo-??-carbolines obtained by bromination of ??-carboline alkaloids, Journal of Heterocyclic Chemistry, vol.2, issue.5, pp.1087-1096, 2001. ,
DOI : 10.1002/jhet.5570380512
New Alkaloids, Picrasidines W, X and Y, from Picrasma quassioides and X-Ray Crystallographic Analysis of Picrasidine Q., CHEMICAL & PHARMACEUTICAL BULLETIN, vol.41, issue.10, pp.1807-1811, 1993. ,
DOI : 10.1248/cpb.41.1807
Synthesis and isolation of chloro-??-carbolines obtained by chlorination of ??-carboline alkaloids in solution and in solid state, Journal of Heterocyclic Chemistry, vol.29, issue.3, pp.419-426, 2003. ,
DOI : 10.1002/jhet.5570400304
Total Syntheses of the Chlorinated ?????Carboline Alkaloids Bauerine A, B, and C, Synthetic Communications, vol.48, issue.8, pp.1273-1280, 2007. ,
DOI : 10.3987/COM-98-8107
Points of Significance: Two-factor designs, Nature Methods, vol.11, issue.12, pp.1187-1188, 2014. ,
DOI : 10.1038/nmeth.2858