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Imaging Vesicular Traffic at the Immune Synapse.


Immunological synapse formation is the result of a profound T cell polarization process that involves the coordinated action of the actin and microtubule cytoskeleton, as well as intracellular vesicle traffic. Endosomal vesicle traffic ensures the targeting of the T cell receptor (TCR) and various signaling molecules to the synapse, being necessary for the generation of signaling complexes downstream of the TCR. Here we describe the microscopy imaging methods that we currently use to unveil how TCR and signaling molecules are associated with endosomal compartments and deliver their cargo to the immunological synapse.
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pasteur-01511350 , version 1 (20-04-2017)


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Jérôme Bouchet, Iratxe del Río-Iñiguez, Andrés Alcover. Imaging Vesicular Traffic at the Immune Synapse.. Methods in Molecular Biology (ISSN: 1064-3745, ESSN: 1940-6029), 1584, pp.129-142, 2017, The  Immune  Synapse:  Methods  and  Protocols, 978-1-4939-6881-7 (online ISBN). ⟨10.1007/978-1-4939-6881-7_9⟩. ⟨pasteur-01511350⟩


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