Imaging Vesicular Traffic at the Immune Synapse.

Abstract : Immunological synapse formation is the result of a profound T cell polarization process that involves the coordinated action of the actin and microtubule cytoskeleton, as well as intracellular vesicle traffic. Endosomal vesicle traffic ensures the targeting of the T cell receptor (TCR) and various signaling molecules to the synapse, being necessary for the generation of signaling complexes downstream of the TCR. Here we describe the microscopy imaging methods that we currently use to unveil how TCR and signaling molecules are associated with endosomal compartments and deliver their cargo to the immunological synapse.
Document type :
Book sections
Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01511350
Contributor : Florence Jeannot <>
Submitted on : Thursday, April 20, 2017 - 6:04:45 PM
Last modification on : Tuesday, April 25, 2017 - 9:51:31 AM
Long-term archiving on : Friday, July 21, 2017 - 2:05:01 PM

File

Art Bouchet et al Meth Mol Bio...
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial - ShareAlike 4.0 International License

Identifiers

Collections

Citation

Jérôme Bouchet, Iratxe Del Río-Iñiguez, Andrés Alcover. Imaging Vesicular Traffic at the Immune Synapse.. Methods in Molecular Biology (ISSN: 1064-3745, ESSN: 1940-6029), 1584, pp.129-142, 2017, The  Immune  Synapse:  Methods  and  Protocols, 978-1-4939-6881-7 (online ISBN). ⟨10.1007/978-1-4939-6881-7_9⟩. ⟨pasteur-01511350⟩

Share

Metrics

Record views

542

Files downloads

164