Structural insights into serine-rich fimbriae from Gram-positive bacteria.

Abstract : The serine-rich repeat family of fimbriae play important roles in the pathogenesis of streptococci and staphylococci. Despite recent attention, their finer structural details and precise adhesion mechanisms have yet to be determined. Fap1 (Fimbriae-associated protein 1) is the major structural subunit of serine-rich repeat fimbriae from Streptococcus parasanguinis and plays an essential role in fimbrial biogenesis, adhesion, and the early stages of dental plaque formation. Combining multidisciplinary, high resolution structural studies with biological assays, we provide new structural insight into adhesion by Fap1. We propose a model in which the serine-rich repeats of Fap1 subunits form an extended structure that projects the N-terminal globular domains away from the bacterial surface for adhesion to the salivary pellicle. We also uncover a novel pH-dependent conformational change that modulates adhesion and likely plays a role in survival in acidic environments.
Document type :
Journal articles
Complete list of metadatas

Cited literature [64 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01500608
Contributor : Maya Um <>
Submitted on : Monday, April 3, 2017 - 2:51:54 PM
Last modification on : Thursday, April 19, 2018 - 2:24:03 PM
Long-term archiving on : Tuesday, July 4, 2017 - 12:54:13 PM

File

114 BARDIAUX B.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Collections

Citation

Stéphanie Ramboarina, James A Garnett, Meixian Zhou, Yuebin Li, Zhixiang Peng, et al.. Structural insights into serine-rich fimbriae from Gram-positive bacteria.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2010, 285 (42), pp.32446-57. ⟨10.1074/jbc.M110.128165⟩. ⟨pasteur-01500608⟩

Share

Metrics

Record views

118

Files downloads

225