Abstract : The gene dnph1 (or rcl) encodes a hydrolase that cleaves the 2'-deoxyribonucleoside 5'-monophosphate (dNMP) N-glycosidic bond to yield a free nucleobase and 2-deoxyribose 5-phosphate. Recently, the crystal structure of rat DNPH1, a potential target for anti-cancer therapies, suggested that various analogs of AMP may inhibit this enzyme. From this result, we asked whether N (6)-substituted AMPs, and among them, cytotoxic cytokinin riboside 5'-monophosphates, may inhibit DNPH1. Here, we characterized the structural and thermodynamic aspects of the interactions of these various analogs with DNPH1. Our results indicate that DNPH1 is inhibited by cytotoxic cytokinins at concentrations that inhibit cell growth.
https://hal-pasteur.archives-ouvertes.fr/pasteur-01472730 Contributor : Marie MartinConnect in order to contact the contributor Submitted on : Tuesday, February 21, 2017 - 11:23:34 AM Last modification on : Monday, May 16, 2022 - 5:44:28 PM Long-term archiving on: : Monday, May 22, 2017 - 2:40:13 PM