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Journal articles
Pf155/RESA protein influences the dynamic microcirculatory behavior of ring-stage Plasmodium falciparum infected red blood cells.
Abstract : Proteins exported by Plasmodium falciparum to the red blood cell (RBC) membrane modify the structural properties of the parasitized RBC (Pf-RBC). Although quasi-static single cell assays show reduced ring-stage Pf-RBCs deformability, the parameters influencing their microcirculatory behavior remain unexplored. Here, we study the dynamic properties of ring-stage Pf-RBCs and the role of the parasite protein Pf155/Ring-Infected Erythrocyte Surface Antigen (RESA). Diffraction phase microscopy revealed RESA-driven decreased Pf-RBCs membrane fluctuations. Microfluidic experiments showed a RESA-dependent reduction in the Pf-RBCs transit velocity, which was potentiated at febrile temperature. In a microspheres filtration system, incubation at febrile temperature impaired traversal of RESA-expressing Pf-RBCs. These results show that RESA influences ring-stage Pf-RBCs microcirculation, an effect that is fever-enhanced. This is the first identification of a parasite factor influencing the dynamic circulation of young asexual Pf-RBCs in physiologically relevant conditions, offering novel possibilities for interventions to reduce parasite survival and pathogenesis in its human host.
Cited literature [34 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01472091
Contributor : Serge Bonnefoy <>
Submitted on : Monday, February 20, 2017 - 3:17:33 PM
Last modification on : Monday, January 13, 2020 - 5:08:06 PM
Long-term archiving on: : Sunday, May 21, 2017 - 2:21:27 PM
Contributor : Serge Bonnefoy <>
Submitted on : Monday, February 20, 2017 - 3:17:33 PM
Last modification on : Monday, January 13, 2020 - 5:08:06 PM
Long-term archiving on: : Sunday, May 21, 2017 - 2:21:27 PM
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srep00614.pdf
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