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Journal articles
The RAG2 C-terminus and ATM protect genome integrity by controlling antigen receptor gene cleavage.
Abstract : Tight control of antigen-receptor gene rearrangement is required to preserve genome integrity and prevent the occurrence of leukaemia and lymphoma. Nonetheless, mistakes can happen, leading to the generation of aberrant rearrangements, such as Tcra/d-Igh inter-locus translocations that are a hallmark of ataxia telangiectasia-mutated (ATM) deficiency. Current evidence indicates that these translocations arise from the persistence of unrepaired breaks converging at different stages of thymocyte differentiation. Here we show that a defect in feedback control of RAG2 activity gives rise to bi-locus breaks and damage on Tcra/d and Igh in the same T cell at the same developmental stage, which provides a direct mechanism for generating these inter-locus rearrangements. Both the RAG2 C-terminus and ATM prevent bi-locus RAG-mediated cleavage through modulation of three-dimensional conformation (higher-order loops) and nuclear organization of the two loci. This limits the number of potential substrates for translocation and provides an important mechanism for protecting genome stability.
Keywords :
*Gene Rearrangement
alpha-Chain T-Cell Antigen Receptor
*Gene Rearrangement
delta-Chain T-Cell Antigen Receptor
*Genomic Instability
Animals
Ataxia Telangiectasia Mutated Proteins/*metabolism
DNA-Binding Proteins/*chemistry/*metabolism
Genetic Loci
Mice
Receptors
Antigen
T-Cell
alpha-beta/genetics
https://hal-pasteur.archives-ouvertes.fr/pasteur-01471697
Contributor : Anne Lassailly-Bondaz Connect in order to contact the contributor
Submitted on : Monday, February 20, 2017 - 2:40:45 PM
Last modification on : Tuesday, October 19, 2021 - 10:27:50 PM
Long-term archiving on: : Sunday, May 21, 2017 - 2:10:48 PM
Contributor : Anne Lassailly-Bondaz Connect in order to contact the contributor
Submitted on : Monday, February 20, 2017 - 2:40:45 PM
Last modification on : Tuesday, October 19, 2021 - 10:27:50 PM
Long-term archiving on: : Sunday, May 21, 2017 - 2:10:48 PM
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ncomms3231.pdf
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