Genomic characterization of Sebokele virus 1 (SEBV1) reveals a new candidate species among the genus Parechovirus.

Marie-Line Joffret (1) , Christiane Bouchier (2) , Marc Grandadam (3, 4) , Hervé Zeller (5) , Corinne Maufrais (6) , Hervé Bourhy (7, 8) , Philippe Desprès (4) , Francis Delpeyroux (1) , Laurent Dacheux (8, 7)

1 BVE - Biologie des virus entériques
2 Génomique (Plate-Forme) - Genomics Platform
3 Centre national de Référence et Centre Collaborateur de l'OMS Arbovirus (CNR-CCOMS)
4 Interactions Moléculaires Flavivirus-Hôtes
5 UBIVE - Biologie des Infections Virales Émergentes - Biology of Emerging Viral Infections
6 Centre d'Informatique pour la Biologie
7 CNR - Centre National de Référence de la Rage-Dynamique des Lyssavirus et adaptation à l'hôte
8 CC-OMS - Centre Collaborateur de l'OMS pour la Rage - Dynamique des lyssavirus et adaptation à l'hôte

We determined the genomic features and the taxonomic classification of Sebokele virus 1 (SEBV1), a previously unclassified arbovirus isolated in 1972 from rodents collected in Botambi, Central African Republic. The complete genome sequence was obtained using a deep sequencing approach (Illumina technology) and dedicated bioinformatics workflows for data analysis. Molecular analysis identified SEBV1 as a picornavirus, most closely related to Ljungan viruses of the genus Parechovirus. The genome has a typical Ljungan virus-like organization, including the presence of two unrelated 2A protein motifs. Phylogenetic analysis confirmed that SEBV1 belongs to the parechovirus phylogroup and was most closely related to the Ljungan virus species. However, it appeared clearly distinct from all members of this phylogroup, suggesting that it represents a novel species of the genus Parechovirus.

Mots clés: 36 Illumina sequencing; high throughput sequencing; Ljungan virus; complete genome; phylogenetic analysis; Sebokele virus 1; picornavirus; parechovirus

Domaines: Sciences du Vivant [q-bio]; Virologie

Marie-Line Joffret, Christiane Bouchier, Marc Grandadam, Hervé Zeller, Corinne Maufrais, et al.. Genomic characterization of Sebokele virus 1 (SEBV1) reveals a new candidate species among the genus Parechovirus.. Journal of General Virology, 2013, 94 (Pt_7), pp.1547-53. ⟨10.1099/vir.0.053157-0⟩. ⟨pasteur-01435337⟩