Identification of Leishmania-specific protein phosphorylation sites by LC-ESI-MS/MS and comparative genomics analyses

Sonia Hem 1 Pier Federico Gherardini 2 José Fortéa 3 Veronique Hourdel 1 Miguel A. Morales 4, 5 Reiko Watanabe 4, 5 Pascale Pescher 4, 5 Michael A. Kuzyk 6 Derek Smith 6 Christoph H. Borchers 6 Dan Zilberstein 7 Manuela Helmer-Citterich 2 Abdelkader Namane 1 Gerald F Späth 4, 5, *
* Auteur correspondant
1 Protéomique (Plate-Forme)
2 Centre for Molecular Bioinformatics
3 Immunophysiologie et Parasitisme Intracellulaire
4 Virulence Parasitaire
5 Inserm Avenir Group
6 Genome British Columbia Proteomics Centre [Victoria]
7 Faculty of Biology
Abstract : Human pathogenic protozoa of the genus Leishmania undergo various developmental transitions during the infectious cycle that are triggered by changes in the host environment. How these parasites sense, transduce, and respond to these signals is only poorly understood. Here we used phosphoproteomic approaches to monitor signaling events in L. donovani axenic amastigotes, which may be important for intracellular parasite survival. LC-ESI-MS/MS analysis of IMAC-enriched phosphoprotein extracts identified 445 putative phosphoproteins in two independent biological experiments. Functional enrichment analysis allowed us to gain insight into parasite pathways that are regulated by protein phosphorylation and revealed significant enrichment in our data set of proteins whose biological functions are associated with protein turn-over, stress response, and signal transduction. LC-ESI-MS/MS analysis of TiO(2)-enriched phosphopeptides confirmed these results and identified 157 unique phosphopeptides covering 181 unique phosphorylation sites in 126 distinct proteins. Investigation of phosphorylation site conservation across related trypanosomatids and higher eukaryotes by multiple sequence alignment and cluster analysis revealed L. donovani-specific phosphoresidues in highly conserved proteins that share significant sequence homology to orthologs of the human host. These unique phosphorylation sites reveal important differences between host and parasite biology and post-translational protein regulation, which may be exploited for the design of novel anti-parasitic interventions.
Keywords : Molecular Sequence Data Leishmania Life Cycle Stages Mice Amino Acid Sequence Animals Cells Cultured Chromatography Liquid Cluster Analysis Databases Protein Electrophoresis Gel Two-Dimensional Fungal Proteins Humans Phosphoproteins Proteomics Sequence Alignment Spectrometry Mass Electrospray Ionization Tandem Mass Spectrometry
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Proteomics, Wiley-VCH Verlag, 2010, 10 (21), pp.3868-3883. 〈10.1002/pmic.201000305〉
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Soumis le : jeudi 12 janvier 2017 - 17:10:01
Dernière modification le : jeudi 11 janvier 2018 - 06:21:35

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Sonia Hem, Pier Federico Gherardini, José Fortéa, Veronique Hourdel, Miguel A. Morales, et al.. Identification of Leishmania-specific protein phosphorylation sites by LC-ESI-MS/MS and comparative genomics analyses. Proteomics, Wiley-VCH Verlag, 2010, 10 (21), pp.3868-3883. 〈10.1002/pmic.201000305〉. 〈pasteur-01433567〉

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