Simultaneous multi-parametric analysis of Leishmania and of its hosting mammal cells: A high content imaging-based method enabling sound drug discovery process

Claire-Lise Forestier; Gerald F Späth; Eric Prina; Sreekanth Dasari

Simultaneous multi-parametric analysis of Leishmania and of its hosting mammal cells: A high content imaging-based method enabling sound drug discovery process

Claire-Lise Forestier ^{1, *} Gerald F Späth ² Eric Prina ² Sreekanth Dasari ¹

* Corresponding author

1 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes

2 Parasitologie moléculaire et Signalisation

Abstract : Leishmaniasis is a vector-borne disease for which only limited therapeutic options are available. The disease is ranked among the six most important tropical infectious diseases and represents the second-largest parasitic killer in the world. The development of new therapies has been hampered by the lack of technologies and methodologies that can be integrated into the complex physiological environment of a cell or organism and adapted to suitable in vitro and in vivo Leishmania models. Recent advances in microscopy imaging offer the possibility to assess the efficacy of potential drug candidates against Leishmania within host cells. This technology allows the simultaneous visualization of relevant phenotypes in parasite and host cells and the quantification of a variety of cellular events. In this review, we present the powerful cellular imaging methodologies that have been developed for drug screening in a biologically relevant context, addressing both high-content and high-throughput needs. Furthermore, we discuss the potential of intra-vital microscopy imaging in the context of the anti-leishmanial drug discovery process.

Keywords : Drug Evaluation Animal Animals Antiprotozoal Agents Cytological Techniques Disease Models Preclinical Drug screening Leishmania Microscopy Microscopy imaging Optical Imaging

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Microbiology and Parasitology

Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01433404
Contributor : Marie Martin <>
Submitted on : Thursday, January 12, 2017 - 4:29:56 PM
Last modification on : Thursday, February 7, 2019 - 4:16:02 PM

Simultaneous multi-parametric analysis of Leishmania and of its hosting mammal cells: A high content imaging-based method enabling sound drug discovery process

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Simultaneous multi-parametric analysis of Leishmania and of its hosting mammal cells: A high content imaging-based method enabling sound drug discovery process

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