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Promyelocytic Leukemia Protein (PML) Controls Listeria monocytogenes Infection.

Résumé : The promyelocytic leukemia protein (PML) is the main organizer of stress-responsive subnuclear structures called PML nuclear bodies. These structures recruit multiple interactors and modulate their abundance or their posttranslational modifications, notably by the SUMO ubiquitin-like modifiers. The involvement of PML in antiviral responses is well established. In contrast, the role of PML in bacterial infection remains poorly characterized. Here, we show that PML restricts infection by the pathogenic bacterium Listeria monocytogenes but not by Salmonella enterica serovar Typhimurium. During infection, PML undergoes oxidation-mediated multimerization, associates with the nuclear matrix, and becomes de-SUMOylated due to the pore-forming activity of the Listeria toxin listeriolysin O (LLO). These events trigger an antibacterial response that is not observed during in vitro infection by an LLO-defective Listeria mutant, but which can be phenocopied by specific induction of PML de-SUMOylation. Using transcriptomic and proteomic microarrays, we also characterized a network of immunity genes and cytokines, which are regulated by PML in response to Listeria infection but independently from the listeriolysin O toxin. Our study thus highlights two mechanistically distinct complementary roles of PML in host responses against bacterial infection.
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Submitted on : Thursday, January 12, 2017 - 2:28:36 PM
Last modification on : Wednesday, September 23, 2020 - 4:34:42 AM

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David Ribet, Valérie Lallemand-Breitenbach, Omar Ferhi, Marie-Anne Nahori, Hugo Varet, et al.. Promyelocytic Leukemia Protein (PML) Controls Listeria monocytogenes Infection.. mBio, American Society for Microbiology, 2017, 8 (1), pp.1-16. ⟨10.1128/mBio.02179-16⟩. ⟨pasteur-01433079⟩

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