Design of a Specific Colonic Mucus Marker Using a Human Commensal Bacterium Cell Surface Domain

Yves-Marie S Coïc (1) , Francoise S Baleux (1) , Ömer S Poyraz (2) , Roman Thibeaux (3, 4) , Elisabeth Labruyere (4, 3) , Fabrice Chretien (5) , Iradj S Sobhani (6) , Thierry Lazure (4) , Benjamin S Wyplosz (7, 4) , Gunter S Schneider (2) , Laurence S Mulard (1) , Philippe Sansonetti (7, 8) , Benoit S Marteyn (7)

1 Chimie des biomolécules
2 Department of Medical Biochemistry and Biophysics
3 Biologie Cellulaire du Parasitisme
4 Service d'Anatomo-Pathologie
5 Histopathologie humaine et Modèles animaux
6 Service de gastro-entérologie [Henri Mondor AP-HP, Créteil]
7 Pathogénie Microbienne Moléculaire
8 Collège de France - Chaire Microbiologie et Maladies infectieuses

Background: Imaging colonic mucus on living cells, tissues, and organs is required for live microscopy. Results: We have identified, synthesized, and validated a new human colonic mucus bacterial marker (MUB 70). Conclusion: This non-toxic marker is used to image the secreted colonic mucus. Significance: Beyond imaging applications, Cy5-MUB 70 might be used for diagnostic and prognosis applications on colonic mucinous carcinoma.

Domains: Life Sciences [q-bio]; Bioengineering; Imaging; Chemical Sciences; Life Sciences [q-bio]; Microbiology and Parasitology; Bacteriology

Yves-Marie S Coïc, Francoise S Baleux, Ömer S Poyraz, Roman Thibeaux, Elisabeth Labruyere, et al.. Design of a Specific Colonic Mucus Marker Using a Human Commensal Bacterium Cell Surface Domain. Journal of Biological Chemistry, 2012, ⟨10.1074/jbc.M111.310003⟩. ⟨pasteur-01423132⟩