Identification of a Region That Assists Membrane Insertion and Translocation of the Catalytic Domain of Bordetella pertussis CyaA Toxin

Johanna C. Karst (1) , Robert Barker (2) , Usha Devi (3) , Marcus J. Swann (3) , Marilyne Davi (1) , Stephen J. Roser (4) , Daniel Ladant (1) , Alexandre Chenal (1)

1 Biochimie des Interactions Macromoléculaires / Biochemistry of Macromolecular Interactions
2 DS/LSS Large Scale Structures group
3 Farfiels Group Limited
4 Chemistry

Background: Translocation of the CyaA toxin across plasma membrane is still poorly understood. Results: The region 375– 485 is involved in membrane destabilization in vitro and required for cell intoxication. Conclusion: The region 375– 485 is crucial for membrane insertion and translocation of the catalytic domain of CyaA. Significance: These results provide new insights on the early stages of the cell intoxication process.

Keywords: Adenylate Cyclase Toxin; chemistry/genetics/metabolism; Bordetella pertussis/chemistry/; genetics/metabolism

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology

Johanna C. Karst, Robert Barker, Usha Devi, Marcus J. Swann, Marilyne Davi, et al.. Identification of a Region That Assists Membrane Insertion and Translocation of the Catalytic Domain of Bordetella pertussis CyaA Toxin. Journal of Biological Chemistry, 2012, 287 (12), pp.9200 - 9212. ⟨10.1074/jbc.M111.316166⟩. ⟨pasteur-01423063⟩