Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits. - Institut Pasteur Accéder directement au contenu
Article Dans Une Revue Infection and Immunity Année : 2013

Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits.

Résumé

In response to environmental fluctuations or stresses, bacteria can activate transcriptional and phenotypic programs to coordinate an adaptive response. The intracellular pathogen Legionella pneumophila converts from a noninfectious replicative form to an infectious transmissive form when the bacterium encounters alterations in either amino acid concentrations or fatty acid biosynthesis. Here, we report that L. pneumophila differentiation is also triggered by nicotinic acid, a precursor of the central metabolite NAD(+). In particular, when replicative L. pneumophila are treated with 5 mM nicotinic acid, the bacteria induce numerous transmissive-phase phenotypes, including motility, cytotoxicity toward macrophages, sodium sensitivity, and lysosome avoidance. Transcriptional profile analysis determined that nicotinic acid induces the expression of a panel of genes characteristic of transmissive-phase L. pneumophila. Moreover, an additional 213 genes specific to nicotinic acid treatment were altered. Although nearly 25% of these genes lack an assigned function, the gene most highly induced by nicotinic acid treatment encodes a putative major facilitator superfamily transporter, Lpg0273. Indeed, lpg0273 protects L. pneumophila from toxic concentrations of nicotinic acid as judged by analyzing the growth of the corresponding mutant. The broad utility of the nicotinic acid pathway to couple central metabolism and cell fate is underscored by this small metabolite's modulation of gene expression by diverse microbes, including Candida glabrata, Bordetella pertussis, Escherichia coli, and L. pneumophila.
Fichier principal
Vignette du fichier
zii945.pdf (1.04 Mo) Télécharger le fichier
Origine : Publication financée par une institution

Dates et versions

pasteur-01422856 , version 1 (27-12-2016)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Rachel L Edwards, Andrew Bryan, Matthieu Jules, Kaoru Harada, Carmen Buchrieser, et al.. Nicotinic acid modulates Legionella pneumophila gene expression and induces virulence traits.. Infection and Immunity, 2013, 81 (3), pp.945-55. ⟨10.1128/IAI.00999-12⟩. ⟨pasteur-01422856⟩
89 Consultations
162 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More