Characterization of new Salmonella serovars by whole-genome sequencing and traditional typing techniques

Janet Bale; Daniele Meunier; François-Xavier Weill; Elizabeth Depinna; Tansy Peters; Satheesh Nair

Characterization of new Salmonella serovars by whole-genome sequencing and traditional typing techniques

Janet Bale ^{1, *} Daniele Meunier ¹ François-Xavier Weill ² Elizabeth Depinna ¹ Tansy Peters ¹ Satheesh Nair ¹

* Corresponding author

2 CNR-ESS - Centre National de Référence des Escherichia coli, Shigella et Salmonella - Bactéries pathogènes entériques

Abstract : Serotyping forms the basis of all national and international surveillance networks for Salmonella. Public health microbiology is currently being transformed by high-throughput DNA sequencing, which opens the door to serovar determination using this powerful technique. Twenty-nine Salmonella isolates referred to the Public Health England between 1994 and 2004 for serovar identification were selected for this study, and they all presented with novel antigenic formulae. Results from a combination of traditional phenotypic and molecular assays were compared. Twenty-two isolates (76 %) were subsequently independently confirmed as new types; of these, 18 (82 %) were grouped as Salmonellaenterica subspecies I, and four (18 %) were S. enterica subspecies II. In general, it is shown that there is concordance between the DNA sequence type and traditional phenotypic serotype, but it would be necessary to analyse a larger data set to confirm this. Traditional multilocus sequence typing (MLST) by Sanger sequencing also correlates to insilico whole-genome sequencing MLST. This permits the continuation of traditional serovar nomenclature alongside sequence type methods and enhances the ability to infer true phylogenetic relationships between isolates.

Document type :

Journal articles

Domain :

Life Sciences [q-bio]

Life Sciences [q-bio] / Santé publique et épidémiologie

Complete list of metadatas

https://hal-pasteur.archives-ouvertes.fr/pasteur-01421027
Contributor : Marie Lemesle <>
Submitted on : Wednesday, December 21, 2016 - 2:12:47 PM
Last modification on : Friday, February 22, 2019 - 11:02:53 AM
Long-term archiving on : Tuesday, March 21, 2017 - 5:39:18 AM

Characterization of new Salmonella serovars by whole-genome sequencing and traditional typing techniques

Identifiers

Collections

Citation

Export

Metrics

172

Contact

Characterization of new Salmonella serovars by whole-genome sequencing and traditional typing techniques

Identifiers

Collections

Citation

Export

Share

Metrics

172

Contact