Purification of G1 daughter cells from different Saccharomycetes species through an optimized centrifugal elutriation procedure.

Abstract : Centrifugal elutriation discriminates cells according to their sedimentation coefficients, generating homogeneous samples well suited for genomic comparative approaches. It can, for instance, isolate G1 daughter cells from a Saccharomyces cerevisiae unsynchronized population, alleviating ageing and cell-cycle biases when conducting genome-wide/single-cell studies. The present report describes a straightforward and robust procedure to determine whether a cell population of virtually any yeast species can be efficiently elutriated, while offering solutions to optimize success. This approach was used to characterize elutriation parameters and S-phase progression of four yeast species (S. cerevisiae, Candida glabrata, Lachancea kluyveri and Pichia sorbitophila) and could theoretically be applied to any culture of single, individual cells.
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Martial Marbouty, Caroline Ermont, Bernard Dujon, Guy-Franck Richard, Romain Koszul. Purification of G1 daughter cells from different Saccharomycetes species through an optimized centrifugal elutriation procedure.. Yeast, Wiley, 2014, 31 (5), pp.159-166. ⟨10.1002/yea.3005⟩. ⟨pasteur-01420008⟩

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