Generation and Analysis of Chromosomal Contact Maps of Yeast Species.

Axel Cournac (1) , Martial Marbouty (1) , Julien Mozziconacci (2) , Romain Koszul (1)

1 Régulation spatiale des Génomes - Spatial Regulation of Genomes
2 LPTMC - Laboratoire de Physique Théorique de la Matière Condensée

Genome-wide derivatives of the chromosome conformation capture (3C) technique are now well-established approaches to study the multiscale average organization of chromosomes from bacteria to mammals. However, the experimental parameters of the protocol have to be optimized for different species, and the downstream experimental products (i.e., pair-end sequences) are influenced by these parameters. Here, we describe a complete pipeline to generate 3C-seq libraries and compute chromosomal contact maps of yeast species.

Keywords: 3C; 3C analysis; Chromosome conformation capture; Genome assembly; Genome organization; Yeast

Domains: Life Sciences [q-bio]

Axel Cournac, Martial Marbouty, Julien Mozziconacci, Romain Koszul. Generation and Analysis of Chromosomal Contact Maps of Yeast Species.. Methods in Molecular Biology, 2016, Yeast Functional Genomics, 1361, pp.227-45. ⟨10.1007/978-1-4939-3079-1_13⟩. ⟨pasteur-01419912⟩