Skip to Main content Skip to Navigation
Journal articles

Evidence for a dual role of actin in regulating chromosome organization and dynamics in yeast.

Abstract : Eukaryotic chromosomes undergo movements that are involved in the regulation of functional processes such as DNA repair. To better understand the origin of these movements, we used fluorescence microscopy, image analysis and chromosome conformation capture to quantify the actin contribution to chromosome movements and interactions in budding yeast. We show that both the cytoskeletal and nuclear actin drive local chromosome movements, independently of Csm4, a putative LINC protein. Inhibition of actin polymerization reduces subtelomere dynamics, resulting in more confined territories and enrichment in subtelomeric contacts. Artificial tethering of actin to nuclear pores increased both nuclear pore complex (NPC) and subtelomere motion. Chromosome loci that were positioned away from telomeres exhibited reduced motion in the presence of an actin polymerization inhibitor but were unaffected by the lack of Csm4. We further show that actin was required for locus mobility that was induced by targeting the chromatin-remodeling protein Ino80. Correlated with this, DNA repair by homologous recombination was less efficient. Overall, interphase chromosome dynamics are modulated by the additive effects of cytoskeletal actin through forces mediated by the nuclear envelope and nuclear actin, probably through the function of actin in chromatin-remodeling complexes.
Document type :
Journal articles
Complete list of metadatas

Cited literature [74 references]  Display  Hide  Download

https://hal-pasteur.archives-ouvertes.fr/pasteur-01419905
Contributor : Romain Koszul <>
Submitted on : Tuesday, December 20, 2016 - 10:27:37 AM
Last modification on : Wednesday, August 19, 2020 - 11:17:22 AM
Long-term archiving on: : Monday, March 20, 2017 - 10:24:52 PM

File

681full.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Maya Spichal, Alice Brion, Sébastien Herbert, Axel Cournac, Martial Marbouty, et al.. Evidence for a dual role of actin in regulating chromosome organization and dynamics in yeast.. Journal of Cell Science, Company of Biologists, 2016, 129 (4), pp.681-92. ⟨10.1242/jcs.175745⟩. ⟨pasteur-01419905⟩

Share

Metrics

Record views

581

Files downloads

935