Skip to Main content Skip to Navigation
Journal articles

Allelic variation contributes to bacterial host specificity.

Min Yue 1 Xiangan Han 1 Leon de Masi 1 Chunhong Zhu 1 Xun Ma 1 Junjie Zhang 1 Renwei Wu 1 Robert Schmieder 2 Radhey S Kaushik 3 George P Fraser 4 Shaohua Zhao 5 Patrick F Mcdermott 5 François-Xavier Weill 6 Jacques G Mainil 7 Cesar Arze 8 W Florian Fricke 8 Robert A Edwards 9, 2 Dustin Brisson 10 Nancy R Zhang 11 Shelley C Rankin 1 Dieter M Schifferli 1
1 University of Pennsylvania School of Veterinary Medicine
2 SDSU - San Diego State University
3 SDSTATE - South Dakota State University
4 Pennsylvania Department of Health
5 FDA - U.S. Food and Drug Administration
6 BPE - Bactéries pathogènes entériques
7 Université de Liège
8 University of Maryland School of Medicine
9 ANL - Argonne National Laboratory [Lemont]
10 University of Pennsylvania [Philadelphia]
11 The Wharton School
Abstract : Understanding the molecular parameters that regulate cross-species transmission and host adaptation of potential pathogens is crucial to control emerging infectious disease. Although microbial pathotype diversity is conventionally associated with gene gain or loss, the role of pathoadaptive nonsynonymous single-nucleotide polymorphisms (nsSNPs) has not been systematically evaluated. Here, our genome-wide analysis of core genes within Salmonella enterica serovar Typhimurium genomes reveals a high degree of allelic variation in surface-exposed molecules, including adhesins that promote host colonization. Subsequent multinomial logistic regression, MultiPhen and Random Forest analyses of known/suspected adhesins from 580 independent Typhimurium isolates identifies distinct host-specific nsSNP signatures. Moreover, population and functional analyses of host-associated nsSNPs for FimH, the type 1 fimbrial adhesin, highlights the role of key allelic residues in host-specific adherence in vitro. Together, our data provide the first concrete evidence that functional differences between allelic variants of bacterial proteins likely contribute to pathoadaption to diverse hosts.
Document type :
Journal articles
Complete list of metadatas

Cited literature [59 references]  Display  Hide  Download
https://hal-pasteur.archives-ouvertes.fr/pasteur-01419902
Contributor : Marie Lemesle <>
Submitted on : Tuesday, December 20, 2016 - 10:24:22 AM
Last modification on : Thursday, June 18, 2020 - 4:48:05 PM
Long-term archiving on: : Tuesday, March 21, 2017 - 1:42:19 PM

File

Vignette du document
Allelic variation.pdf
Publication funded by an institution

Licence

Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

PASTEUR

Citation

Min Yue, Xiangan Han, Leon de Masi, Chunhong Zhu, Xun Ma, et al.. Allelic variation contributes to bacterial host specificity.. Nature Communications, Nature Publishing Group, 2015, 6 (6), pp.8754. ⟨10.1038/ncomms9754⟩. ⟨pasteur-01419902⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

233

Files downloads

505