An Orthogonal D 2 O-Based Induction System that Provides Insights into d -Amino Acid Pattern Formation by Radical S-Adenosylmethionine Peptide Epimerases - Archive ouverte HAL Access content directly
Journal Articles Angewandte Chemie International Edition Year : 2016

An Orthogonal D 2 O-Based Induction System that Provides Insights into d -Amino Acid Pattern Formation by Radical S-Adenosylmethionine Peptide Epimerases

Abstract

Radical S-adenosyl methionine peptide epimerases (RSPEs) are an enzyme family that accomplishes regiospecific and irreversible introduction of multiple d-configured residues into ribosomally encoded peptides. Collectively, RSPEs can generate diverse epimerization patterns in a wide range of substrates. Previously, the lack of rapid methods to localize epimerized residues has impeded efforts to investigate the function and applicative potential of RSPEs. An efficient mass spectrometry-based assay is introduced that permits characterization of products generated in E. coli. Applying this to a range of non-natural peptide-epimerase combinations, it is shown that the d-amino acid pattern is largely but not exclusively dictated by the core peptide sequence, while the epimerization order is dependent on the enzyme-leader pair. RSPEs were found to be highly promiscuous, which allowed for modular introduction of peptide segments with defined patterns.
Not file

Dates and versions

pasteur-01416272 , version 1 (26-12-2016)

Identifiers

Cite

Brandon I. Morinaka, Marjan Verest, Michael F. Freeman, Muriel Gugger, Jörn Piel. An Orthogonal D 2 O-Based Induction System that Provides Insights into d -Amino Acid Pattern Formation by Radical S-Adenosylmethionine Peptide Epimerases. Angewandte Chemie International Edition, 2016, 55, ⟨10.1002/anie.201609469⟩. ⟨pasteur-01416272⟩

Collections

PASTEUR
116 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More