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Journal articles
An Orthogonal D 2 O-Based Induction System that Provides Insights into d -Amino Acid Pattern Formation by Radical S-Adenosylmethionine Peptide Epimerases
Abstract : Radical S-adenosyl methionine peptide epimerases (RSPEs) are an enzyme family that accomplishes regiospecific and irreversible introduction of multiple d-configured residues into ribosomally encoded peptides. Collectively, RSPEs can generate diverse epimerization patterns in a wide range of substrates. Previously, the lack of rapid methods to localize epimerized residues has impeded efforts to investigate the function and applicative potential of RSPEs. An efficient mass spectrometry-based assay is introduced that permits characterization of products generated in E. coli. Applying this to a range of non-natural peptide-epimerase combinations, it is shown that the d-amino acid pattern is largely but not exclusively dictated by the core peptide sequence, while the epimerization order is dependent on the enzyme-leader pair. RSPEs were found to be highly promiscuous, which allowed for modular introduction of peptide segments with defined patterns.
https://hal-pasteur.archives-ouvertes.fr/pasteur-01416272
Contributor : Bénédicte Benedic Connect in order to contact the contributor
Submitted on : Monday, December 26, 2016 - 3:56:10 PM
Last modification on : Wednesday, December 1, 2021 - 4:56:01 PM
Contributor : Bénédicte Benedic Connect in order to contact the contributor
Submitted on : Monday, December 26, 2016 - 3:56:10 PM
Last modification on : Wednesday, December 1, 2021 - 4:56:01 PM