Phenotypic CD8+ T Cell Diversification Occurs before, during, and after the First T Cell Division

Abstract : Effector T cell responses rely on a phenotypically and functionally heterogeneous population of cells. Whether this diversity is programmed before clonal expansion or in later phases as a result of stochastic events or asymmetric cell division is not fully understood. In this study, we first took advantage of a sensitive in vitro assay to analyze the composition of single CD8(+) T cell progenies. Heterogeneity was predominantly observed between progenies of distinct clones, but could also be detected within individual progenies. Furthermore, by physically isolating daughter cells of the first T cell division, we showed that differences in paired daughter cell progenies contributed to intraclonal diversification. Finally, we developed an in vivo limiting dilution assay to compare individual T cell progenies following immunization. We provided evidence for simultaneous intraclonal and interclonal diversification in vivo. Our results support the idea that T cell diversification is a continuous process, initiated before clonal expansion and amplified during the first and subsequent cell divisions.
Type de document :
Article dans une revue
Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2013, 191 (4), pp.1578 - 1585. 〈10.4049/jimmunol.1300424〉
Liste complète des métadonnées

https://hal-pasteur.archives-ouvertes.fr/pasteur-01410269
Contributeur : Bérengère Hugot <>
Soumis le : mardi 6 décembre 2016 - 15:30:16
Dernière modification le : jeudi 15 décembre 2016 - 01:07:34

Lien texte intégral

Identifiants

Collections

Citation

F. Lemaitre, H. D. Moreau, L. Vedele, P. Bousso. Phenotypic CD8+ T Cell Diversification Occurs before, during, and after the First T Cell Division. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2013, 191 (4), pp.1578 - 1585. 〈10.4049/jimmunol.1300424〉. 〈pasteur-01410269〉

Partager

Métriques

Consultations de la notice

30