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Phenotypic CD8+ T Cell Diversification Occurs before, during, and after the First T Cell Division

Abstract : Effector T cell responses rely on a phenotypically and functionally heterogeneous population of cells. Whether this diversity is programmed before clonal expansion or in later phases as a result of stochastic events or asymmetric cell division is not fully understood. In this study, we first took advantage of a sensitive in vitro assay to analyze the composition of single CD8(+) T cell progenies. Heterogeneity was predominantly observed between progenies of distinct clones, but could also be detected within individual progenies. Furthermore, by physically isolating daughter cells of the first T cell division, we showed that differences in paired daughter cell progenies contributed to intraclonal diversification. Finally, we developed an in vivo limiting dilution assay to compare individual T cell progenies following immunization. We provided evidence for simultaneous intraclonal and interclonal diversification in vivo. Our results support the idea that T cell diversification is a continuous process, initiated before clonal expansion and amplified during the first and subsequent cell divisions.
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https://hal-pasteur.archives-ouvertes.fr/pasteur-01410269
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Submitted on : Tuesday, December 6, 2016 - 3:30:16 PM
Last modification on : Friday, March 27, 2020 - 3:44:21 AM

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F. Lemaitre, H. D. Moreau, L. Vedele, P. Bousso. Phenotypic CD8+ T Cell Diversification Occurs before, during, and after the First T Cell Division. Journal of Immunology, Publisher : Baltimore : Williams & Wilkins, c1950-. Latest Publisher : Bethesda, MD : American Association of Immunologists, 2013, 191 (4), pp.1578 - 1585. ⟨10.4049/jimmunol.1300424⟩. ⟨pasteur-01410269⟩

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