Phenotypic CD8+ T Cell Diversification Occurs before, during, and after the First T Cell Division - Archive ouverte HAL Access content directly
Journal Articles Journal of Immunology Year : 2013

Phenotypic CD8+ T Cell Diversification Occurs before, during, and after the First T Cell Division

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Abstract

Effector T cell responses rely on a phenotypically and functionally heterogeneous population of cells. Whether this diversity is programmed before clonal expansion or in later phases as a result of stochastic events or asymmetric cell division is not fully understood. In this study, we first took advantage of a sensitive in vitro assay to analyze the composition of single CD8(+) T cell progenies. Heterogeneity was predominantly observed between progenies of distinct clones, but could also be detected within individual progenies. Furthermore, by physically isolating daughter cells of the first T cell division, we showed that differences in paired daughter cell progenies contributed to intraclonal diversification. Finally, we developed an in vivo limiting dilution assay to compare individual T cell progenies following immunization. We provided evidence for simultaneous intraclonal and interclonal diversification in vivo. Our results support the idea that T cell diversification is a continuous process, initiated before clonal expansion and amplified during the first and subsequent cell divisions.

Dates and versions

pasteur-01410269 , version 1 (06-12-2016)

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F. Lemaitre, H. D. Moreau, L. Vedele, P. Bousso. Phenotypic CD8+ T Cell Diversification Occurs before, during, and after the First T Cell Division. Journal of Immunology, 2013, 191 (4), pp.1578 - 1585. ⟨10.4049/jimmunol.1300424⟩. ⟨pasteur-01410269⟩
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