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Journal articles
The mechanism of anti-CD20–mediated B cell depletion revealed by intravital imaging
Abstract : Anti-CD20 Ab therapy has proven successful for treating B cell malignancies and a number of autoimmune diseases. However, how anti-CD20 Abs operate in vivo to mediate B cell depletion is not fully understood. In particular, the anatomical location, the type of effector cells, and the mechanism underlying anti-CD20 therapy remain uncertain. Here, we found that the liver is a major site for B cell depletion and that recirculation accounts for the decrease in B cell numbers observed in secondary lymphoid organs. Using intravital imaging, we established that, upon anti-CD20 treatment, Kupffer cells (KCs) mediate the abrupt arrest and subsequent engulfment of B cells circulating in the liver sinusoids. KCs were also effective in depleting malignant B cells in a model of spontaneous lymphoma. Our results identify Ab-dependent cellular phagocytosis by KCs as a primary mechanism of anti-CD20 therapy and provide an experimental framework for optimizing the efficacy of therapeutic Abs.
Cited literature [25 references]
https://hal-pasteur.archives-ouvertes.fr/pasteur-01410108
Contributor : Bérengère Hugot <>
Submitted on : Tuesday, December 6, 2016 - 2:18:08 PM
Last modification on : Wednesday, September 23, 2020 - 12:56:02 PM
Long-term archiving on: : Monday, March 20, 2017 - 7:32:20 PM
Contributor : Bérengère Hugot <>
Submitted on : Tuesday, December 6, 2016 - 2:18:08 PM
Last modification on : Wednesday, September 23, 2020 - 12:56:02 PM
Long-term archiving on: : Monday, March 20, 2017 - 7:32:20 PM
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JCI70972.pdf
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