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Journal articles
Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry
Abstract : Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.
Keywords :
PCR
Polymerase Chain Reaction
Prospective Studies
Culture
DNA
Fungal
electrospray-ionization mass spectrometry
Humans
Microbiological Techniques
Mucorales
Mucorales identification
Mucormycosis
Pathology
Molecular
Real-Time Polymerase Chain Reaction
Sequence Analysis
species level
Spectrometry
Mass
Electrospray Ionization
Time Factors
https://hal-pasteur.archives-ouvertes.fr/pasteur-01405221
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 4:14:23 PM
Last modification on : Wednesday, August 19, 2020 - 11:18:12 AM
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 4:14:23 PM
Last modification on : Wednesday, August 19, 2020 - 11:18:12 AM
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