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Journal articles
Staphylococcus aureus RNAIII and the endoribonuclease III coordinately regulate spa gene expression
Abstract : Staphylococcus aureus RNAIII is one of the largest regulatory RNAs, which controls several virulence genes encoding exoproteins and cell-wall-associated proteins. One of the RNAIII effects is the repression of spa gene (coding for the surface protein A) expression. Here, we show that spa repression occurs not only at the transcriptional level but also by RNAIII-mediated inhibition of translation and degradation of the stable spa mRNA by the double-strand-specific endoribonuclease III (RNase III). The 3' end domain of RNAIII, partially complementary to the 5' part of spa mRNA, efficiently anneals to spa mRNA through an initial loop-loop interaction. Although this annealing is sufficient to inhibit in vitro the formation of the translation initiation complex, the coordinated action of RNase III is essential in vivo to degrade the mRNA and irreversibly arrest translation. Our results further suggest that RNase III is recruited for targeting the paired RNAs. These findings add further complexity to the expression of the S. aureus virulon.
Keywords :
Antisense
Ribosomes
RNA
Antigens
Bacterial
Bacterial Proteins
Base Sequence
Binding Sites
Gene Expression Regulation
Molecular Sequence Data
Nucleic Acid Conformation
Protein Biosynthesis
Ribonuclease III
Messenger
RNA Stability
Staphylococcal Protein A
Staphylococcus aureus
Transcription
Genetic
https://hal-pasteur.archives-ouvertes.fr/pasteur-01404709
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 10:41:39 AM
Last modification on : Tuesday, December 1, 2020 - 10:38:03 AM
Contributor : Floran Bidault <>
Submitted on : Tuesday, November 29, 2016 - 10:41:39 AM
Last modification on : Tuesday, December 1, 2020 - 10:38:03 AM
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